The Cytotoxicity Test and Rejection Observation of Abdominal Zipper for Miniature Pigs

doi:10.3969/j.issn.1674-5817.2013.02.010

Abstract

Abstract: Objective To assess the safety of abdominal zippers of miniature pig by cytotoxicity test and rejection observation. Methods The safety of abdominal zippers of miniature pig were evaluated by CCK-8 cytotoxicity test. In the test of rejection observation, nine miniature pigs were randomly divided into three groups: control group(C group), surgical procedure group(S group) and the animal model of miniature pig with abdominal zipper group(M group), and IL-1, IL-6, IL-10 and TNF-〈 in blood serum were detected before surgery (0 d), and during the day of 1d, 3 d, 7 d, 14 d and 21 d after surgery. Results The cytotoxicity grade was 0 orⅠlevel, and the level of IL-1, IL-6, IL-10 and TNF-〈 in S group and M group increased significantly at 1d and 3 d after surgery, but there was no difference between S group, M group and C group at 7 d, 14 d and 21 d after surgery. Conclusions The abdominal zipper for miniature pigs, which satisfies the national safety standard of biological materials, has qualified cytotoxicity and excellent biosafety.

Key words: Miniature pig, Bio-zipper, Cytotoxicity test, Rejection

CLC Number:

R-332Q95-33

YU Chen-lin, TANG Qing, LIU Zhi-xue, MA Lei, XU Chen, CAI Li-ping, CUI Shu-fang. The Cytotoxicity Test and Rejection Observation of Abdominal Zipper for Miniature Pigs[J]. Laboratory Animal and Comparative Medicine, 2013, 33(2): 130-134.

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