Effect of HSP70 on Fibrosis of Nonalcoholic Fatty Liver Disease in Rabbits

doi:10.3969/j.issn.1674-5817.2014.02.006

Abstract

Abstract: Objective To investigate the change of HSP70 in nonalcoholic fatty (NAFLD) liver fibrosis of rabbit. Methods Forty male Japanese white rabbits were randomly assigned to control group and model group. The model group was divided into three subgroups, as 4-week, 6-week, 8-week groups, with 10 rabbits in each, and treated with peanut oil (1.2 ml/kg) subcutaneously (s.c.), twice a week for 4 weeks, 6 weeks and 8 weeks respectively. The rabbits of all groups were sacrificed at the designed time point. Samples of serum were collected for detecting the serum ALT, AST, TC, TG, FFA, FBG, HSP47, HSP70, HA, PC?, C˘ô, LN ,and the HOMA-IR were calculated. The liver tissues were collected for histopathological and immunohistochemical observation. Results The model groups had hepatic injury and high level of insulin at the beginning of 4 weeks. With the process of NAFLD liver fibrosis, HSP70 had gradually increased. Conclusion The expression of HSP70 was positively correlated with NAFLD liver fibrosis, and it might have close relation with the development of NAFLD liver fibrosis.

Key words: NAFLD, Liver fibrosis, HSP70, HOMA-IR

CLC Number:

Q95-33

DENG Li-li, ZANG Jia-na, YING Hua-zhong, YU Chen-huan, YU Bing. Effect of HSP70 on Fibrosis of Nonalcoholic Fatty Liver Disease in Rabbits[J]. Laboratory Animal and Comparative Medicine, 2014, 34(2): 107-112.

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