Expression Characteristics of miR-504 in Oral Squamous Cell Carcinoma of Chinese Hamster

doi:10.3969/j.issn.1674-5817.2019.05.002

Abstract

Abstract: Objective An animal model was established to study the expression characteristics of miR-504 in the cancer and adjacent paracancerous tongue tissues of the Chinese hamster oral squamous cell carcinoma (OSCC). Methods The OSCC model of Chinese hamster was induced by smear of 9,10-Dimethy1-1,2-Benzanthracence (DMBA), cloning and analysis of the precursor sequence of miR-504 in OSCC. The total miRNAs in the cheek pouch and paracancerous tongue tissues were detected by qRT-PCR at different time points (0, 6, 12 and 18 weeks of modeling). Results The buccal mucosa showed mild hyperemia at 6 week, thickened at 12 week, local leukoplakia lesions and erosion at 18 week. Compared with the normal control group, the expression levels of miR-504 in the cheek pouch tissue increased by about 0.83, 0.33 and 0.23 times (P<0.001) at 6, 12, and 18 weeks respectively. The expression level in the tongue tissue was about 0.92, 0.84 and 0.27 times compared with that of normal tongue tissue respectively (0.001<P<0.01). Conclusions The expression of miR-504 was significantly increased in the OSCC model established by DMBA smear of the hamster cheek pouch tissue. miR-504 is closely related to the development of OSCC and may regulate its target gene and its downstream signaling pathway. The results of this study are helpful to understand the differential expression characteristics of miR-504 in the cancer and paracancerous groups of various stages of OSCC in Chinese hamsters.

Key words: Chinese hamster, Oral squamous cell carcinoma (OSCC), MiR-504 expression characteristics

CLC Number:

Q95-33

WEI Jia-ning, XU Guo-qiang, GAO Ji-ping, WANG Xiao-tang, XIAO Lan-fei, SONG Guo-hua. Expression Characteristics of miR-504 in Oral Squamous Cell Carcinoma of Chinese Hamster[J]. Laboratory Animal and Comparative Medicine, 2019, 39(5): 342-348.

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