Observation on Oxidative Stress in Chronic Myocardial Ischemia in Mini-pig

doi:10.3969/j.issn.1674-5817.2012.05.006

Abstract

Abstract: Objective To observe the effects of oxidative stress on chronic myocardial ischemia in mini-pigs. Methods Fifty-five Bama mini-pigs were randomly divided into three groups: normal control (Ctr) group, high-fat (HF) group and chronic myocardial ischemia (CMI) group, five animals in each group. The chronic myocardial ischemia model was made induced by vitamin D₃, isoproterenol and high-fat diet for 24 weeks. The changes of phosphokinase isoenzyme (CK-MB), platelet aggregation rate (PAR) and the SOD, MDA, GSH-Px, NO, LD, ATPase in myocardial tissues were detected. Results Compared with normal control group, the serum CK-MB activity, PAR, the myocardial tissues of MDA and LD contents in CMI group were significant increased (P<0.05, P<0.01), the myocardial tissues of SOD, SOD/MDA ratio, GSH-Px, NO, Na⁺-K⁺-ATPase, Ca⁺⁺-ATPase and Mg⁺⁺-ATPase in CMI group were lowered (P<0.05, P<0.01). PAR and the myocardial tissues of SOD/MDA ratio and MDA content in HF group were significant increased (P<0.05, P<0.01), while the myocardial tissues of SOD, GSH-Px, Na⁺-K⁺-ATPase and Mg⁺⁺-ATPase were significant decreased (P<0.05). Compared with HF group, the serum CK-MB activity in CMI group was significant higher than that of HF group (P<0.01) and the myocardial tissues of Na⁺-K⁺-ATPase and Ca⁺⁺-ATPase were significant lower than those of HF group (P<0.05, P<0.01). Correlation analysis showed that there was a positive correlation between CK-MB and PAR, MDA in CMI group (P<0.05, P<0.01), and a significant negative correlation between CK-MB and SOD, SOD/MDA, GSH-Px, NO respectively(P<0.05, P<0.01). The platelet aggregation rate was positively correlated with CK-MB and MDA (P<0.05), and was negative relation with SOD, SOD/MDA and Na⁺-K⁺-ATPase (P<0.05, P<0.01). There was a significant positively correlation between LD and MDA in myocardial tissues (P<0.05), and LD was negatively correlated with SOD/MDA, Na⁺-K⁺-ATPase, Ca⁺⁺-ATPase and Mg⁺⁺-ATPase (P<0.05). NO level was positively correlated with SOD, SOD/MDA, GSH-Px in myocardial tissues(P<0.05, P<0.01), and was negatively related with CK-MB and MDA (P<0.05). Conclusion Oxidative stress injury was participated in the formation of chronic myocardial ischemia.

Key words: Oxidative stress, Chronic myocardial ischemia, Mini-pig

CLC Number:

Q95-33

XU Xiao-ping, PAN Yong-ming, CHEN Liang, XU Jian-qin, WANG De-jun, ZHU Ke-yan, YANG Yu-wei, CHEN Min-li. Observation on Oxidative Stress in Chronic Myocardial Ischemia in Mini-pig[J]. Laboratory Animal and Comparative Medicine, 2012, 32(5): 401-406.

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