Abstract: Objective To research the effect of YIGUTANG containing serum on osteoblast proliferation which was from the skull of new born Wistar rat in vitro, and IL-6 secretion. Methods Iliac bone specimens were obtained from the newborn rat calvaria, after that the bone pieces were digested by collagenase-trypsin. The proliferation of osteoblast was observed using MTT reduction assay. The IL-6 in the supernatant was detected by ELISA. Results The proliferation of osteoblast could be stimulated by the serum with different dosages of absorbed YIGUTANG (group A 、B 、C). Meanwhile, the levels of IL-6 secretion in group A, B and C could be significantly decreased compared with the control group (P<0.05). Conclusion The proliferation of osteoblast can be stimulated by the serum with absorbed YIGUTANG and the capability of producing IL-6 in osteoblast also can be restrained by the serum.

Key words: YIGUTANG containing serum, Osteoblast, Proliferation, Interleukin-6