Comparative Study on Immunological Functions of Intestina Parva Between WHBE Rabbit and Japanese White Rabbit

doi:10.3969/j.issn.1674-5817.2012.02.009

Abstract

Abstract: Objective To investigate differences on immunological function of small bowel between WHBE rabbit and Japanese white rabbit. Methods Sixteen WHBE rabbit and Sixteen Japanese white rabbit were used and each group at different stage was 8. Levels of secretory IgA (SIgA), Substance P (SP), IL-4, IFN-γ, somatostatin (SS) and Vasoactive Intestinal Peptide (VIP) in serum and small bowel were determined by ELISA. Results The levels of sIgA, SS、SP and the ratio of IFN-γ/IL-4 in serum and small bowel of grown WHBE rabbit were much lower than those of weanling WHBE rabbit. In particular, the levels of sIgA, SS, IFN-γ and IL-4 in small bowel of grown WHBE rabbit lower than those of grown JW rabbit. Conclusions Immunological function of WHBE Rabbit was much weaker than that of Japanese Rabbit. It may warrant further evaluation as a possible animal for the research of irritable bowel syndrome.

Key words: WHBE Rabbit, Japanese white rabbit, Immunological function, Small bowel, Growth stage

CLC Number:

Q95-33

WANG Zhi-yuan, LIU Yue-huan, YU Chen-huan, YING Hua-zhong, CAI yue-qin, Zhang li-zong. Comparative Study on Immunological Functions of Intestina Parva Between WHBE Rabbit and Japanese White Rabbit[J]. Laboratory Animal and Comparative Medicine, 2012, 32(2): 125-128.

References

[1] Lv JM, Chen ML, Qian LC, et al.Requirement of crude protein for maintenance in a new strain of laboratory rabbit[J]. Animal Feed Science and Technology, 2009, 151(3-4):261-267.
[2] 陈民利, 赵伟春, 应华忠, 等. WHBE兔血液蛋白的特性[J].中国比较医学杂志, 2009, 19(11):51-58.
[3] 徐孝平, 徐剑钦, 张利棕, 等. WHBE兔对卵白蛋白诱发变应性鼻炎的敏感性研究[J]. 临床和实验医学杂志, 2011, 10(12):881-883.
[4] 艾秀峰, 冷晓霞, 陈民利, 等. 心理应激致亚健康状态大鼠的免疫功能改变与中药干预的影响[J]. 实验动物与比较医学, 2010, 30(2):117-120.
[5] 潘永明, 张利棕, 陈民利, 等. WHBE兔脾虚型肠易激综合征模型的建立[J].实验动物与比较医学, 2008, 28(5):313-317.
[6] Grover M, Drossman DA.Centrally acting therapies for irritable bowel syndrome[J]. Gastroenterology Clinics of North America, 2011, 40(1):183-206.
[7] 刘忠华, 王颖谚, 黄韧, 等. 禽流感H5N1型病毒对沙鼠致病性的初步研究[J].实验动物与比较医学, 2005, 25(4):199-202.
[8] 朱雪萍, 陈卫昌, 许诤, 等. 肠易激综合症幼鼠模型的建立及初步探讨[J]. 实验动物与比较医学, 2007, 27(3):172-175.
[9] 逄利, 臧秀贤, 吴扬. 神经-免疫-内分泌网络在肠易激综合征发病中的作用[J]. 2010, 13(1):334-336.
[10] Clarke G, Quigley EMM, Cryan JF, et al.Irritable bowel syndrome: towards biomarker identification[J]. Trends in Molecular Medicine, 2009, 15(10):478-489.
[11] Kraneveld AD, Rijnierse A, Nijkamp FP, et al.Neuro-immune interactions in inflammatory bowel disease and irritable bowel syndrome: Future therapeutic targets[J]. Eur J Pharmacol, 2008, 585(2-3):361-374.
[12] 银新, 宁守斌. 肠易激综合征发病机制的研究进展[J]. 世界华人消化杂志, 2009, 17(32): 3318-3323.
[13] 赵佳钧, 李宏宇, 孙大为. 腹泻型肠易激综合征患者外周血Th1/Th2细胞因子失衡表达的分析[J]. 细胞与分子免疫学杂志, 2011,27(2):201-202.

[1]	ZHOU Yan, DIAO Chen-xi, ZHANG Yuan-yuan, YU Hai-bo, LU Tao-feng, ZHAO Li-li, CHEN Hong-yan. Composition and Diversity of Fecal Microflora in SPF Chickens at Different Growth Stages [J]. Laboratory Animal and Comparative Medicine, 2017, 37(3): 231-239.
[2]	CHEN Fang-ming, LIN Lin, CAI Yue-qin, CHEN Cheng, CHEN Min-li. The Forelimb Bone Mineral Density and Expression of Calcitonin and Osteocalcin in Forelimb Malformed White Hair & Black Eyes Rabbits [J]. Laboratory Animal and Comparative Medicine, 2015, 35(5): 383-389.
[3]	WANG Feng, HAO Fei, HUAN Hong-hua, ZHANG Yuan-peng, ZHANG Bo, SHAO Guo-qing. Study on Changes of Body Temperature in Japanese White Rabbit and New Zealand Rabbit under Physiological Conditions [J]. Laboratory Animal and Comparative Medicine, 2015, 35(3): 222-226.
[4]	PAN Yong-ming, CHEN Liang, XU Jian-qin, CHEN Min-li. Study on Relation between Cardiovascular System and Cardiac Autonomic Nervous Function in WHBE Rabbits Myocardial Ischemia Model of Phlegm-blood Stasis Syndrome Type [J]. Laboratory Animal and Comparative Medicine, 2015, 35(2): 161-166.
[5]	XU Wen-mang, LI Xia, HE Zhan-long, LI Tao, ZHAO Xi-long, WANG Yuan-yuan, DAI Fang, LIU Qiang-gao, CAI Lin, PAN Xin-yan, YANG Ju-lun. Study on Comparative Histology of Digestive Glands in 6 Species of Laboratory Animals [J]. Laboratory Animal and Comparative Medicine, 2014, 34(6): 454-462.
[6]	WANG Yuan-yuan, XU Wen-mang, LI Xia, LI Tao, DAI Fang, YANG Ju-lun, SONG Shu-ling, LIU Qiang-gao. Comparison on Histology of Cardiovascular System in 6 Species of Laboratory Animals [J]. Laboratory Animal and Comparative Medicine, 2014, 34(3): 199-204.
[7]	HU Hui-ying, WANG Zhi-yuan, KE Xiang-fu, YU Chen-huan, TU Jue, ZHANG Li-zhong, YING Hua-zhong. Comparative Study on Immunological Functions of Intestina Parva Between WHBE Rabbit and Japanese White Rabbit [J]. Laboratory Animal and Comparative Medicine, 2013, 33(3): 215-217.
[8]	TU Jue, XU Jian-qin, PAN Yong-ming, LIU Zhi-hua, KONG Qin, CHEN Min-li. Comparation of Dendritic Cells from Different Sources of WHBE Rabbits [J]. Laboratory Animal and Comparative Medicine, 2012, 32(3): 209-214.
[9]	LU Ting, PAN Yong-ming, CHEN Min-li, CHEN Liang, XU Jian-qin, HE Huan, Wang De-jun, YING Hua-zhong. Observation and Comparasion on Cardiac Hemodynamics in WHBE Rabbit with Acute Myocardial Ischemia [J]. Laboratory Animal and Comparative Medicine, 2011, 31(4): 264-268.
[10]	XU Xiao-ping, PAN Yong-ming, CHEN Liang, HE Huan, XU Jian-qin, ZHU Ke-yan, YING Hua-zhong, CHEN Min-li. Comparative Study on Myocardial Ischemia Model Induced by Ligation of Coronary Artery in WHBE Rabbits and Japanese White Rabbits [J]. Laboratory Animal and Comparative Medicine, 2011, 31(2): 87-91.
[11]	YING Hua-zhong, SHOU Qi-yang, CHEN Min-li, PAN Yong-ming,WANG Hui, LIN Lin, ZHU Ke-yan, WANG De-jun, XU Xiao-ping. Determination and Comparison of Hematological Indices in WHBE Rabbits [J]. , 2010, 30(1): 44-47.
[12]	YING Hua-zhong, ZHU Ke-yan，CHEN Mm-li，SHOU Qi-yang, LV Jian-mm, PAN Yong-ming, . Comparasion on Blood Biochemical Indices among Three Breeds of Rabbit [J]. , 2009, 29(6): 407-410.
[13]	ZHOU Wei-mm, PAN Yong-mmg, CHEN Mm-li，WANG Hui, LIN Lm, WANG De-jun . Measurement of Reticulocytes and Its Subpopulation in WHBE Rabbits [J]. , 2009, 29(6): 411-414.
[14]	SHOU Qi-yang, CHEN Mm-li, ZHU Ke-yan, PAN Yong-mmg, PEN Dmg-guo, WANG Hui, LIN Lin, LV Jian-min. Analysis and Determination of Hemorheology and Blood Coagulation Function in WHBE Rabbits [J]. , 2009, 29(6): 415-418.
[15]	CHEN Liang, PAN Yong-mmg, CHEN Mm-li，ZHANG Li-zong, XU Jian-qm, YING Hua-zhong. Determination and Analysis of Electrocardiogram in Anesthetized WHBE Rabbits [J]. , 2009, 29(6): 419-423.