Effect of XUEBIJING on Multiple Organ Dysfunction Syndrome Induced by Lipopolysaccharide in Sheep

doi:10.3969/j.issn.1674-5817.2012.02.008

Abstract

Abstract: Objective To investigate the prevention and cure efficacy of XUEBIJING on multiple organ dysfunction syndrome (MODS) induced by lipopolysaccharide (LPS) in sheep. Methods Fourty sheep were randomly divided into 4 groups: the control (Group A), model (Group B), precaution (Group C) and cure group (Group D), 10 sheep each group . After anesthesia, the MODS model was established by infusing LPS at 6 μg/kg within 30 minutes intravenously. Then the sheep were infused with XUEBIJING (2 ml/kg) by intravenous drip before and 1 hour after injecting LPS in Group C and Group D respectively; animals in Group D received XUNBEJING (2 ml/kg) by intravenous drip once daily for 3 days. Meanwhile, the sheep were infused with isotonic Na chloride at the same dose in Group A. The indexes of blood gas, blood routine and blood biochemistry were determined at the different time points. Results After intravenous injecting with LPS, the physical sign was alleviative to some extent in Group C and Group D than that in Group B. The death rates of sheep were 0%, 60%, 40% and 50% in Group A , Group B, Group C and Group D respectively. Compared to the values of oxygenation indexes prior to injection with LPS, it decreased obviously (P<0.05 or P<0.01) within 3 hours in Group B, Group C and Group D, but there were not significant statistical differences observed between three groups (P>0.05). At the different time points, the level of aspartic transaminase (AST), alanine aminotransferase (ALT), total bilirubin (TBIL) and creatinine (CRE) were obviously increased compared to that prior to injection (P<0.05 or P<0.01) . But the number of white blood cell (WBC) was obviously decreased at 3 h~6 h, then it began to increase at 12 h and increased significantly at 24 h (P< 0.01). Conclusion XUEBIJING could improve physical sign appearance, degrade the death rate of MODS induced by LPS and it had interventional effect on damage in multiple organ .

Key words: XUEBIJING, Multiple organ dysfunction syndrome(MODS), Lipopolysaccharide (LPS), Prevention and cure

CLC Number:

Q95-33

SHI Zhi-yong, LUO Xiao-hong, WAN Dong-jun, LU Lu, WANG Hong-yi, NIU Ting-xian. Effect of XUEBIJING on Multiple Organ Dysfunction Syndrome Induced by Lipopolysaccharide in Sheep[J]. Laboratory Animal and Comparative Medicine, 2012, 32(2): 120-124.

References

[1] Marshall JC.SIRS and MODS : What is their relevance to the science and practice of intensive care[J]. Shock, 2000, 14(6):586-589.
[2] 张畔, 曹书华, 崔克亮, 等. 血必净对多脏器功能障碍综合征单核细胞 HLA2DR表达影响的研究[J]. 中国中西医结合急救杂志, 2002, 9(1):21-23.
[3] 曹书华, 王今达. 血必净对感染性多器官功能障碍综合征大鼠组织及内皮损伤保护作用的研究[J]. 中国危重病急救医学, 2002, 14(8):489-491.
[4] 郭凤梅, 邱海波, 周韶霞, 等. 急性呼吸窘迫综合征绵羊模型的建立[J]. 中国危重病急救医学, 2001, 13(2):95-98.
[5] 牛廷献, 罗晓红, 万东君, 等. 内毒素致绵羊急性呼吸窘迫综合症多脏器的病理学改变[J]. 实验动物与比较医学, 2011, 31(4):248-251.
[6] 胡森, 盛志勇, 周宝桐, 等. 双相迟发多器官功能不全综合征(MODS)动物模型的研究[J]. 中华创伤杂志, 1996, 12:102-106.
[7] Beruard G R, Artigas A, Brigham K L, et al.The American-European Consensus Conference on ARDS: definitions, mechanism, relevant, outcomes, and clinical trial coordination[J]. Am J Respir Crit Care Med, 1994, 149:818-824.
[8] 段绍斌, 李学雄, 姚华, 等. 多器官功能障碍综合征时血管内皮细胞损伤的研究[J]. 新疆医科大学学报, 2010, 33(3):271-274.
[9] 卢瑗瑗, 尹文, 虎晓岷. 家兔失血性休克并内毒素诱发多器官功能障碍综合征模型制备[J]. 江苏医药, 2005, 31(6):448-450.
[10] 任添华, 牛驰, 任爱民, 等. MODS动物模型组织氧代谢的变化特征[J].首都医科大学学报, 2008, 29(3):308-310.
[11] Nolan B, Collette H, Baker S, et al.Inhibition of neutrophil apoptosis after severe trauma is NF kappabeta dependent[J]. Traum, 2000, 48(4):599-605.
[12] 王占科, 杨莉萍, 褚芳, 等. 严重创伤多器官功能不全综合症大鼠模型建立[J]. 实验动物与比较医学, 2005, 25(4):218-232.
[13] 胡森, 盛志勇, 周宝桐. MODS动物模型研究进展[J]. 中国危重病急救医学, 1999,11(8):504-507.
[14] Mustered RA, Fisher J, Hayman S, et al.Cardiopulmonary response to pseudomon as septicemia in swine: an improved model of the adultre spiratory distress syndrome[J]. Lab Animal Sci, 1989, 39(1):37-43.
[15] 曹书华, 王今达, 李银平. 从“菌毒并治”到“四证四法”–关于中西医结合治疗多器官功能障碍综合征辨证思路的深入与完善[J]. 中国危重病急救医学, 2005, 17(11):641-643.
[16] 马巍, 杨季红, 马静, 等. 血必净对大鼠肝缺血–再灌注后急性肺损伤的保护作用[J]. 河北医药, 2008, 30(2):142-143.
[17] 马俊清, 路伟, 黄杨, 等. 血必净注射液对百草枯刺激的大鼠肺微血管内皮细胞损伤的保护作用[J]. 中国急救医学, 2008, 28(9):808-812.
[18] 林福清, 邓小明, 朱科明. “血必净”注射液对急性炎症反应综合征患者中性粒细胞功能的影响[J]. 同济大学学报(医学版), 2007, 28(6):86-89.
[19] 张淑文, 孙成栋, 文燕, 等. 血必净注射液对脓毒症大鼠血清内毒素及脾脏特异性免疫功能的影响[J]. 中国中西医结合急救杂志, 2007, 14(2):91-94.
[20] 曹书华, 王今达. 血必净对感染性多器官功能障碍综合征大鼠组织及内皮损伤保护作用的研究[J]. 中国危重病急救医学, 2002, 14(8):489-491.
[21] 王强, 姚咏明, 王文江, 等. 血必净注射液对烫伤大鼠肺组织高迁移率族蛋白B1表达的影响及意义[J]. 解放军医学杂志, 2007, 32(4):374-376.
[22] 刘达兴, 卢斌, 梁贵友, 等. 静脉滴注血必净对体外循环肺损伤的保护作用研究[J]. 中国药房, 2008, 19(24):1860-1862.
[23] 张云杰. 血必净注射液对小肠缺血再灌注大鼠血清细胞因子的影响[J]. 山东中医药大学学报, 2008, 32(6):502-505.
[24] 袁雪松, 卞晓星, 戚传平. 血必净对大鼠外伤性脑损伤后血清S-100β和IL-6 水平的影响[J]. 江苏大学学报(医学版), 2009, 19(1):35-38.
[25] 孙军平, 汪建新, 郭丽娜, 等. 内毒素致兔急性肺损伤及血必净保护作用[J]. 军医进修学院学报, 2009, 30(1):102-104.
[26] 高红梅, 常文秀, 曹书华. “血必净”注射液对内毒素刺激的内皮细胞的影响[J]. 中国急救医学, 2005, 25(6):437-438.
[27] 孙正达, 沈建明, 沈蕾. 血必净注射液改善脓毒症大鼠心脏功能的作用[J]. 复旦大学学报(医学版), 2008, 35(2):208-211.
[28] 任添华, 姜悦, 任爱民, 等. 单相速发型多脏器功能障碍综合征动物模型的研究[J]. 首都医科大学学报, 2005, 26(4):479-481.
[29] 平易, 刘骏峰, 谢琼红, 等. 内毒素介导的多脏器功能障碍综合症动物模型的研究[J]. 中国临床医学, 2009, 16(2):287-290.