Composition and Diversity of Fecal Microflora in SPF Chickens at Different Growth Stages

doi:10.3969/j.issn.1674-5817.2017.03.013

Laboratory Animal and Comparative Medicine ›› 2017, Vol. 37 ›› Issue (3): 231-239.DOI: 10.3969/j.issn.1674-5817.2017.03.013

Special Issue: 实验动物资源开发与利用

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Composition and Diversity of Fecal Microflora in SPF Chickens at Different Growth Stages

ZHOU Yan^1,2, DIAO Chen-xi^1,3, ZHANG Yuan-yuan¹, YU Hai-bo¹, LU Tao-feng¹, ZHAO Li-li¹, CHEN Hong-yan¹

1. Division of Laboratory Animal and Comparative Medicine, State Key Laboratory of Veterinary Biotechnology/Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences, Harbin 150069, China;
2. College of Life Science, Northeast Agricultural University, Harbin 150030, China;
3. College of Life Science and Technology, Mudanjiang Normal University, Mu Danjiang 157011, China

Received:2017-03-16 Online:2017-06-25 Published:2017-06-25

Abstract

Abstract: Objective To provide the composition and diversity of intestinal flora at different growth stages of SPF chickens. Methods The feces of SPF chickens at 10 d , 12 weeks , 23 weeks and 52 weeks old were collected. The two hypervariable regions (V3-V4) of the 16S rRNA of the samples were sequenced using the Illumina HiSeq 2500 platform, and the alpha diversity, beta diversity and fecal community characteristics were analyzed. Results The fecal flora sample at four time points had similar high abundance and good uniformity and there was no significant difference in species diversity between the two groups (P>0.05). More than 95% of abundance were found in Firmicutes, Proteobacteria and Bacteroidetes; Lactobacillus, Streptococcus, Bacteroides, and Enterococcus were dominant species, and the abundance of different periods were significantly different (P<0.05). Conclusions The feces flora of SPF chiken at different growth stages are diverse, and the dominant bacteria are mainly beneficial bacteria. The experimental results are expected to provide the basis for assessing the quality, health monitoring and safety evaluation of SPF chickens.

Key words: SPF chicken, Different growth stages, Feces microbiota, 16s rRNA, Illumina HiSeq 2500 platform

CLC Number:

Q95-33

ZHOU Yan, DIAO Chen-xi, ZHANG Yuan-yuan, YU Hai-bo, LU Tao-feng, ZHAO Li-li, CHEN Hong-yan. Composition and Diversity of Fecal Microflora in SPF Chickens at Different Growth Stages[J]. Laboratory Animal and Comparative Medicine, 2017, 37(3): 231-239.

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Composition and Diversity of Fecal Microflora in SPF Chickens at Different Growth Stages

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