Establishment of Efficient Embryo Transfer System in Transgenic Rabbit and Cloned Rabbit production

doi:10.3969/j.issn.1674-5817.2011.03.004

Abstract

Abstract: Objective To establish an efficient platform of embryo transplantation for production of transgenic rabbits through virus transduction and somatic nuclear transfer approaches. Method Fertilized embryos, transgenic embryos, and nuclear transfer embryos were surgically transplanted into oviduct of synchronous or asynchronous estrus rabbits. Result After transplantation with fertilized embryos, transgenic embryos, and nuclear transfer embryos, the pregnant rates of surrogates were 100%, 66.67% and 36.36%, with the birth of 19, 24 and 17 pups. Survival rates of the pups delivered by recipients with fertilized embryos, transgenic embryos, and nuclear transfer embryos were 90.48%, 92.31% and 17.65%, meantime, the ratios of delivered pub to transferred embryos were 43.75%, 21.31% and 8.54%, respectively. Conclusion An efficient rabbit embryo transfer platform was successfully established, which would be the foundation of production of transgenic rabbits and cloned rabbits.

Key words: Rabbit, Embryo transplantation, Superovulation, Fertilized embryo, Transgenic embryo, Nuclear transfer embryo

CLC Number:

R-33Q95-33

LI Hai-bin, TIAN Jiang-tian, SONG Jun, LAI Liang-xue, GU Wei-wang. Establishment of Efficient Embryo Transfer System in Transgenic Rabbit and Cloned Rabbit production[J]. Laboratory Animal and Comparative Medicine, 2011, 31(3): 172-177.

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