Investigation and Discussion on the Standardization Work of Feeds for Laboratory Animals

doi:10.12300/j.issn.1674-5817.2022.019

Abstract

Abstract:

Standardization of laboratory animals is the basis for obtaining accurate and stable experimental data, drawing objective and reliable experimental conclusions. The standardization of laboratory animal feeds is an integral part of laboratory animal standardization and an important support to realize laboratory animal standardization. In this paper, we compared the historical process of laboratory animal feeds standardization at home and abroad, outlined the key problems existing in the current domestic work, and put forward targeted countermeasures in order to provide references for the standardization of laboratory animal feeds in China.

Key words: Laboratory animal, Feeds, Standardization

CLC Number:

Q95-33

Jianbo LUO, Junhui LI, Jia ZHOU, Yong HE, Min FU. Investigation and Discussion on the Standardization Work of Feeds for Laboratory Animals[J]. Laboratory Animal and Comparative Medicine, 2022, 42(5): 458-465.

Figures/Tables 1

References 31

1	周正宇. 针对生物医学类研究生实验动物学教学的思考[J]. 实验动物与比较医学, 2020, 40(6):537-539. DOI:10.3969/j.issn.1674-5817.2020.06.014 .
	ZHOU Z Y. Thoughts on laboratory animal science teaching for biomedical graduate students[J]. Lab Animal Comp Med, 2020, 40(6):537-539. DOI:10.3969/j.issn.1674-5817.2020.06.014 .
2	韩帅, 朱晓伟, 包思成, 等. 实验动物饲料质量检测体系建设初探[J]. 中国饲料, 2019(9):10-14. DOI:10.15906/j.cnki.cn11-2975/s.20190903 .
	HAN S, ZHU X W, BAO S C, et al. Study on the quality testing system of experiment animal feed[J]. China Feed, 2019(9):10-14. DOI:10.15906/j.cnki.cn11-2975/s.20190903 .
3	National Research Council. Nutrient requirements of laboratory animals[M].4th ed. Washington, DC: National Academy Press, 1995:1-58. DOI: 10.1086/419223 .
4	REEVES P G, NIELSEN F H, FAHEY G C. AIN-93 purified diets for laboratory rodents: final report of the American institute of nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet[J]. J Nutr, 1993, 123(11):1939-1951. DOI:10.1093/jn/123.11.1939 .
5	高诚. «实验动物与比较医学»创刊40年重要文献回顾[J]. 实验动物与比较医学, 2021, 41(1):17-26. DOI:10.12300/j.issn.1674-5817.2020.201 .
	GAO C. Important literature review for the 40th anniversary of Laboratory Animal and Comparative Medicine[J]. Lab Animal Comp Med, 2021, 41(1):17-26. DOI:10.12300/j.issn.1674-5817.2020.201 .
6	史良, 张海峰. 实验动物饲料营养特点及质量影响因素[J]. 实验动物科学, 2019, 36(6):69-72. DOI:10.3969/j.issn.1006-6179.2019.06.014 .
	SHI L, ZHANG H F. Nutrition characteristics and quality influencing factors of laboratory animals feed[J]. Lab Animal Sci, 2019, 36(6):69-72. DOI:10.3969/j.issn.1006-6179.2019.06.014 .
7	KLURFELD D M, GREGORY J F, FIOROTTO M L. Should the AIN-93 rodent diet formulas be revised?[J]. J Nutr, 2021, 151(6):1380-1382. DOI:10.1093/jn/nxab041 .
8	闫立新, 刘跃昌, 寇红岩.国内外实验动物饲料标准化的探讨[C]. 成都: 中国西部第十二届实验动物管理与学术研讨会论文集, 2013:274-277.
	YAN L, LIU Y, KOU H. Discussion on the standardization of experimental animal feed at home and abroad [C]. Chengdu: Proceedings of the 12th Western China Laboratory Animal Management and Academic Seminar, 2013:274-277.
9	CAO Z P, ZHANG T Y, XU C Y, et al. AIN-93 diet as an alternative model to lieber-DeCarli diet for alcoholic cardiomyopathy[J]. Alcohol Clin Exp Res, 2019, 43(7):1452-1461. DOI:10.1111/acer.14069 .
10	罗建波, 李军晖, 王海江, 等. 高脂饲料诱导代谢性疾病动物模型[J]. 实验动物与比较医学, 2021, 41(1):70-78. DOI:10.12300/j.issn.1674-5817.2021.050 .
	LUO J B, LI J H, WANG H J, et al. Metabolic disease animal models induced by high-fat diets[J]. Lab Animal Comp Med, 2021, 41(1):70-78. DOI: 10.12300/j.issn.1674-5817.2021.050 .
11	SPEAKMAN J R. Use of high-fat diets to study rodent obesity as a model of human obesity[J]. Int J Obes, 2019, 43(8):1491-1492. DOI:10.1038/s41366-019-0363-7 .
12	刘源. 实验动物饲料标准化初探[J]. 实验动物科学与管理, 1997, 14(2):30-33.
	LIU Y. Preliminary study on standardization of experimental animal feed[J]. Lab Animal Sci Adm, 1997, 14(2):30-33.
13	王江莉. 数学建模思维在饲料配方问题中的实践探索: 评«蛋鸡饲料配方设计»[J]. 饲料研究, 2019, 42(6):3.
	WANG J L. Practical exploration of mathematical modeling thinking in feed formula—comment on Feed Formula Design for Laying Hens[J]. Feed Res, 2019, 42(6):3.
14	张鑫, 韩蓓, 胡俊涛, 等. 豆粕替代鱼粉对乌鳢生长性能、蛋白质利用及肠道组织形态的影响[J]. 动物营养学报, 2020, 32(6):2799-2807.
	ZHANG X, HAN B, HU J T, et al. Effects of replacement of fish meal by soybean meal on growth performance, protein utilization and intestinal tissue morphology of Channa argus [J]. Chin J Animal Nutr, 2020, 32(6):2799-2807.
15	钟兴文, 杨秀娟, 章雨竹, 等. 优质肉鸡的能量需要量研究进展[J]. 饲料研究, 2019, 42(6):119-125. DOI:10.13557/j.cnki.issn1002-2813.2019.06.033 .
	ZHONG X W, YANG X J, ZHANG Y Z, et al. Research progress on energy metabolism and energy requirement of high-quality broilers[J]. Feed Res, 2019, 42(6):119-125. DOI:10.13557/j.cnki.issn1002-2813.2019.06.033 .
16	SPEAKMAN J R. Body size, energy metabolism and lifespan[J]. J Exp Biol, 2005, 208(Pt 9):1717-1730. DOI:10.1242/jeb.01556 .
17	HE P, JIANG W D, LIU X G, et al. Dietary biotin deficiency decreased growth performance and impaired the immune function of the head kidney, spleen and skin in on-growing grass carp (Ctenopharyngodon idella)[J]. Fish Shellfish Immunol, 2020, 97:216-234. DOI:10.1016/j.fsi.2019.12.033 .
18	ROHR M W, NARASIMHULU C A, RUDESKI-ROHR T A, et al. Negative effects of a high-fat diet on intestinal permeability: a review[J]. Adv Nutr, 2020, 11(1):77-91. DOI:10.1093/advances/nmz061 .
19	LUO J B, FENG L, JIANG W D, et al. The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine[J]. Fish Shellfish Immunol, 2014, 40(1):197-207. DOI:10.1016/j.fsi.2014. 07.003 .
20	王金明, 霍丽娟. 常见植物性饲料中抗营养因子的危害分析[J]. 国外畜牧学(猪与禽), 2011, 31(1):77-79. DOI:10.3969/j.issn.1001-0769.2011.01.038 .
	WANG J M, HUO L J. Hazard analysis of anti-nutritional factors in common plant feed[J]. Animal Sci Abroad Pigs Poult, 2011, 31(1):77-79. DOI: 10.3969/j.issn.1001-0769.2011.01.038 .
21	SONNENBURG E D, SMITS S A, TIKHONOV M, et al. Diet-induced extinctions in the gut microbiota compound over generations[J]. Nature, 2016, 529(7585):212-215. DOI:10.1038/nature16504 .
22	CHASSAING B, MILES-BROWN J, PELLIZZON M, et al. Lack of soluble fiber drives diet-induced adiposity in mice[J]. Am J Physiol Gastrointest Liver Physiol, 2015, 309(7):G528-G541. DOI:10.1152/ajpgi.00172.2015 .
23	陈丙波. 大鼠蛋白质营养需要研究[J]. 北京实验动物科学, 1993, 10(2):35-37.
	CHEN B B. Study on nutritional needs of protein in rats[J]. Lab Animal Sci Adm, 1993, 10(2):35-37.
24	陈小莉, 蔡东联, 李燕, 等. 高蛋氨酸饮食对大鼠生长及氨基酸代谢的影响[J]. 中国临床营养杂志, 2002, 10(4): 248-251. DOI:10.3760/cma.j.issn.1674-635X.2002.04.007 .
	CHEN X L, CAI D L, LI Y, et al. The impact of high methionine diet on the growth and the amino acid metabol ism of rats[J]. Chin J Chinical Nutr, 2002, 10(4): 248-251. DOI:10.3760/cma.j.issn.1674-635X.2002.04.007 .
25	罗培林, 郑萍, 何军, 等. 不同硒源及硒水平对大鼠生长性能、血清抗氧化能力和组织硒沉积的影响[J]. 动物营养学报, 2012, 24(7):1311-1319. DOI:10.3969/j.issn.1006-267x.2012.07.016 .
	LUO P L, ZHENG P, HE J, et al. Different selenium sources and levels affect growth performance, serum antioxidant ability and tissue selenium retention in wistar rats[J]. Chin J Animal Nutr, 2012, 24(7):1311-1319. DOI:10.3969/j.issn.1006-267x.2012.07.016 .
26	赵丹, 王朝旭, 孙长颢, 等. 维生素A缺乏对大鼠铁营养状况的影响[J]. 中国公共卫生, 2003, 19(9):1067-1068. DOI:10.11847/zgggws2003-19-09-27 .
	ZHAO D, WANG C X, SUN C H, et al. Effect of vitamin A deficiency on iron nutritional status in rats[J]. China Public Health, 2003, 19(9):1067-1068. DOI:10.11847/zgggws2003-19-09-27 .
27	王连生, 范泽, 吴迪, 等. 水产动物蛋氨酸营养研究进展[J]. 动物营养学报, 2020, 32(11):4981-4991. DOI:10.3969/j.issn.1006-267x.2020.11.001 .
	WANG L S, FAN Z, WU D, et al. Research progress of methionine nutrition in aquatic animals[J]. Chin J Animal Nutr, 2020, 32(11):4981-4991. DOI:10.3969/j.issn.1006-267x.2020.11.001 .
28	张克连, 郭静宜, 戴若竹. 虾青素对ApoE基因敲除小鼠动脉粥样硬化的降脂、抗氧化和抗炎作用研究[J]. 中国免疫学杂志, 2020, 36(7):794-798. DOI:10.3969/j.issn.1000-484X.2020.07.006 .
	ZHANG K L, GUO J Y, DAI R Z. Effects of astaxanthin on lipid-lowering, anti-oxidation and anti-inflammation of athero-sclerosis in ApoE knockout mice[J]. Chin J Immunol, 2020, 36(7):794-798. DOI:10.3969/j.issn.1000-484X.2020.07.006 .
29	刘云波. 一种免疫缺陷小鼠饲料及其制备工艺: CN112244151A[P]. 2021-01-22.
	LIU Y B. An immunodeficiency mice feed and its preparation process: CN112244151A[P]. 2021-01-22.
30	吴小马, 程良平, 石蕊. 一种免疫缺陷鼠饲料及其制备方法: CN112704153A[P]. 2021-04-27.
	WU X M, CHENG L P, SHI R. An immunodeficiency mice feed and its preparation: CN112704153A[P]. 2021-04-27.
31	刘平怀, 高玉梅, 左遨勋, 等. 一种免疫缺陷鼠饲料及其制备方法: CN113243460A[P]. 2021-08-13.
	LIU P H, GAO Y M, ZUO A X, et al. An immunodeficiency mice feed and its preparation: CN113243460A[P]. 2021-08-13.

营养成分 Nutrient	需要量（每千克饲料含量）/g Requirements (content in 1 kg feeds )/g
营养成分 Nutrient	维持阶段 Maintenance stage	生长阶段 Growth stage	繁殖阶段（雌性） Reproductive stage (Female)
脂肪 Fat	50.0	50.0	50.0
亚油酸（n-6) Linoleic acid	-	6.0	3.0
蛋白质 Protein	50.0	150.0	150.0
氨基酸 Amino acids
精氨酸 Arginine	-	4.3	4.3
苯丙氨酸+酪氨酸 Phenylalanine + Tyrosine	1.9	10.2	10.2
组氨酸 Histidine	0.8	2.8	2.8
赖氨酸 Lysine	1.1	9.2	9.2
异亮氨酸 Isoleucine	3.1	6.2	6.2
亮氨酸 Leucine	1.8	10.7	10.7
蛋氨酸+半胱氨酸 Methionine + Cystine	2.3	9.8	9.8
苏氨酸 Threonine	1.8	6.2	6.2
色氨酸 Tryptophan	0.5	2.0	2.0
缬氨酸 Valine	2.3	7.4	7.4
矿物质 Mineral
钙 Calcium	5.0	5.0	6.3
氯化物 Chloride	0.5	0.5	0.5
镁 Magnesium	0.5	0.5	0.6
磷 Phosphorus	3.0	3.0	3.7
钾 Potassium	3.6	3.6	3.6
钠 Sodium	0.5	0.5	0.5
铜 Copper	5.0×10^-3	5.0×10^-3	8.0×10^-3
铁 Iron	3.5×10^-2	3.5×10^-2	7.5×10^-2
锰 Manganese	1.0×10^-2	1.0×10^-2	1.0×10^-2
锌 Zinc	1.2×10^-2	1.2×10^-2	2.5×10^-2
碘 Iodine	1.5×10^-4	1.5×10^-4	1.5×10^-4
钼 Molybdenum	1.5×10^-4	1.5×10^-4	1.5×10^-4
硒 Selenium	1.5×10^-4	1.5×10^-4	4.0×10^-4
维生素 Vitamin
维生素A Retinol	7.0×10^-4	7.0×10^-4	7.0×10^-4
维生素D Cholecalciferol	2.5×10^-5	2.5×10^-5	2.5×10^-5
维生素E RRR-α-tocopherol	1.8×10^-2	1.8×10^-2	1.8×10^-2
维生素K Phylloquinone	1.0×10^-3	1.0×10^-3	1.0×10^-3
生物素 Biotin	2.0×10^-4	2.0×10^-4	2.0×10^-4
胆碱 Choline	0.75	0.75	0.75
叶酸 Folic acid	1.0×10^-3	1.0×10^-3	1.0×10^-3
烟酸 Niacin	1.5×10^-2	1.5×10^-2	1.5×10^-2
泛酸 Pantothenate	1.0×10^-2	1.0×10^-2	1.0×10^-2
核黄素 Riboflavin	3.0×10^-3	3.0×10^-3	4.0×10^-3
硫胺素 Thiamin	4.0×10^-3	4.0×10^-3	4.0×10^-3
维生素B6 Pyridoxine	6.0×10^-3	6.0×10^-3	6.0×10^-3
维生素B12 Cobalamine	5.0×10^-5	5.0×10^-5	5.0×10^-5

营养成分 Nutrient	需要量（每千克饲料含量）/g Requirements (content in 1 kg feeds )/g
营养成分 Nutrient	维持阶段 Maintenance stage	生长阶段 Growth stage	繁殖阶段（雌性） Reproductive stage (Female)
脂肪 Fat	50.0	50.0	50.0
亚油酸（n-6) Linoleic acid	-	6.0	3.0
蛋白质 Protein	50.0	150.0	150.0
氨基酸 Amino acids
精氨酸 Arginine	-	4.3	4.3
苯丙氨酸+酪氨酸 Phenylalanine + Tyrosine	1.9	10.2	10.2
组氨酸 Histidine	0.8	2.8	2.8
赖氨酸 Lysine	1.1	9.2	9.2
异亮氨酸 Isoleucine	3.1	6.2	6.2
亮氨酸 Leucine	1.8	10.7	10.7
蛋氨酸+半胱氨酸 Methionine + Cystine	2.3	9.8	9.8
苏氨酸 Threonine	1.8	6.2	6.2
色氨酸 Tryptophan	0.5	2.0	2.0
缬氨酸 Valine	2.3	7.4	7.4
矿物质 Mineral
钙 Calcium	5.0	5.0	6.3
氯化物 Chloride	0.5	0.5	0.5
镁 Magnesium	0.5	0.5	0.6
磷 Phosphorus	3.0	3.0	3.7
钾 Potassium	3.6	3.6	3.6
钠 Sodium	0.5	0.5	0.5
铜 Copper	5.0×10^-3	5.0×10^-3	8.0×10^-3
铁 Iron	3.5×10^-2	3.5×10^-2	7.5×10^-2
锰 Manganese	1.0×10^-2	1.0×10^-2	1.0×10^-2
锌 Zinc	1.2×10^-2	1.2×10^-2	2.5×10^-2
碘 Iodine	1.5×10^-4	1.5×10^-4	1.5×10^-4
钼 Molybdenum	1.5×10^-4	1.5×10^-4	1.5×10^-4
硒 Selenium	1.5×10^-4	1.5×10^-4	4.0×10^-4
维生素 Vitamin
维生素A Retinol	7.0×10^-4	7.0×10^-4	7.0×10^-4
维生素D Cholecalciferol	2.5×10^-5	2.5×10^-5	2.5×10^-5
维生素E RRR-α-tocopherol	1.8×10^-2	1.8×10^-2	1.8×10^-2
维生素K Phylloquinone	1.0×10^-3	1.0×10^-3	1.0×10^-3
生物素 Biotin	2.0×10^-4	2.0×10^-4	2.0×10^-4
胆碱 Choline	0.75	0.75	0.75
叶酸 Folic acid	1.0×10^-3	1.0×10^-3	1.0×10^-3
烟酸 Niacin	1.5×10^-2	1.5×10^-2	1.5×10^-2
泛酸 Pantothenate	1.0×10^-2	1.0×10^-2	1.0×10^-2
核黄素 Riboflavin	3.0×10^-3	3.0×10^-3	4.0×10^-3
硫胺素 Thiamin	4.0×10^-3	4.0×10^-3	4.0×10^-3
维生素B6 Pyridoxine	6.0×10^-3	6.0×10^-3	6.0×10^-3
维生素B12 Cobalamine	5.0×10^-5	5.0×10^-5	5.0×10^-5

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