Biosafety Problems and Countermeasures in the Management of Barrier Facilities for Laboratory Animals

doi:10.12300/j.issn.1674-5817.2021.164

Abstract

Abstract:

Biosafety is an important part of national security, and effective control of biological hazards of laboratory animals is the basis of using laboratory animals. Based on the experience of biosafety management in the Laboratory Animal Center of Shanghai General Hospital, this paper analyzed the potential biosafety threats faced by laboratory animal barrier facilities in the process of ensuring the smooth conducting of animal experiments from the perspective of a laboratory animal facility manager, and discussed the relevant countermeasures to reduce such risks, in order to provide a reference for the proper control of biosafety problems in animal experiments.

Key words: Laboratory animals, Biosafety, Facility management, Problems and countermeasures

CLC Number:

Q95-33

Yuqin YANG. Biosafety Problems and Countermeasures in the Management of Barrier Facilities for Laboratory Animals[J]. Laboratory Animal and Comparative Medicine, 2022, 42(2): 95-101.

Figures/Tables 1

References 25

1	李秀, 黎宁, 杨寿清, 等. 屏障系统内实验动物饲养管理中的生物安全、动物福利与伦理[J]. 上海畜牧兽医通讯, 2013(5):46-47. DOI:10.3969/j.issn.1000-7725.2013.05.024 .
2	WOOLHOUSE M E J, GOWTAGE-SEQUERIA S. Host range and emerging and reemerging pathogens[J]. Emerg Infect Dis, 2005, 11(12):1842-1847. DOI:10.3201/eid1112. 050997 .
3	CUNNINGHAM A A, DASZAK P, WOOD J L N. One Health, emerging infectious diseases and wildlife: two decades of progress?[J]. Philos Trans R Soc Lond B Biol Sci, 2017, 372(1725):20160167. DOI:10.1098/rstb.2016.0167 .
4	王锡乐, 巩薇, 贺争鸣, 等. 中国大陆地区实验动物使用现状分析[J]. 实验动物科学, 2017, 34(6):42-48, 54. DOI:10.3969/j.issn.1006-6179.2017.06.010 .
5	罗银珠, 闵凡贵, 王静, 等. 病原微生物防控在实验动物设施管理与生物安全控制中的作用探讨[J]. 实验动物与比较医学, 2021, 41(5):443-449. DOI:10.12300/j.issn.1674-5817. 2020.219 .
6	马春峰, 郭振东, 汤文庭. 动物生物安全实验室常见生物危害及控制措施[J]. 畜牧与兽医, 2019, 51(9):119-124.
7	WANG W L, QI W J, LIU J Y, et al. First human infection case of monkey B virus identified in China, 2021[J]. China CDC Wkly, 2021, 3(29):632-633. DOI:10.46234/ccdcw2021.154 .
8	张强, 熊炜, 李健, 等. 裸鼠过度角化症牛棒状杆菌的分离鉴定[J]. 中国预防兽医学报, 2011, 33(8):581-584. DOI:10.3969/j.issn.1008-0589.2011.08.01 .
9	杨国淋, 李阳友, 苏畅, 等. 实验动物屏障系统建设与管理探讨[J]. 现代农业科技, 2017(16):236-237, 241. DOI:10.3969/j.issn.1007-5739.2017.16.142 .
10	李春秋, 孙艺洋, 王景伟, 等.高校实验动物生物安全管理与建设[J]. 特种经济动植物, 2020, 23(11):8-12.
11	董智强, 罗雷, 王玉林, 等. 广州市某高校动物实验室生物安全事件的调查与分析[J]. 医学动物防制, 2010, 26(1):14-15, 18.
12	李凯航, 杨显超, 吴秀娟, 等. 三种人畜共患病在多种动物中的血清学调查[J]. 上海畜牧兽医通讯, 2018(1):29-31. DOI:10.14170/j.cnki.cn31-1278/s.2018.01.011 .
13	TRIMMEL N E, WALZER C. Infectious wildlife diseases in Austria-A literature review from 1980 until 2017[J]. Front Vet Sci, 2020, 7:3. DOI:10.3389/fvets.2020.00003 .
14	汪诚信. 实验动物饲养场所的害鼠治理[J]. 中国媒介生物学及控制杂志, 2007(2):85-88.
15	XU J X, LI N. Biosafety assessment of genetically engineered animals: a review[J]. Sheng Wu Gong Cheng Xue Bao, 2012, 28(3):267-281.
16	CHOI S A, YUN J W, JOO K M, et al. Preclinical biosafety evaluation of genetically modified human adipose tissue-derived mesenchymal stem cells for clinical applications to brainstem glioma[J]. Stem Cells Dev, 2016, 25(12):897-908. DOI:10.1089/scd.2015.0324 .
17	谢忠忱, 江轶, 黄开胜, 等. 高校实验动物生物安全管理模式研究[J]. 实验技术与管理, 2020, 37(2):1-5. DOI:10.16791/j.cnki.sjg.2020.02.001 .
18	马小琴, 徐鋆娴. 实验动物从业人员职业危害及其防护研究进展[J]. 中国职业医学, 2014, 12(6):735-738. DOI:10.11763/j.issn.2095-2619.2014.06.028 .
19	罗琴, 范才文. 高校动物实验的安全与防护[J]. 华夏医学, 2019, 32(2):163-165. DOI:10.19296/j.cnki.1008-2409.2019-02-051 .
20	刘厚茹, 徐铭. 浅谈医学院校动物实验室生物安全管理与防范[J]. 甘肃畜牧兽医, 2021, 51(07):18-21.
21	李鹏辉, 张宗兴, 徐新喜. 生物安全实验室初级防护设备和废弃物处理设备现状及发展建议[J]. 医疗卫生装备, 2021, 42(5):59-65. DOI:10.19745/j.1003-8868.2021100 .
22	李润林, 董鹏程, 陈靖, 等. 实验动物废弃物无害化处理的现状和对策[J]. 中兽医医药杂志, 2020, 39(6):85-88. DOI:10.13823/j.cnki.jtcvm.2020.06.021 .
23	张晶, 代明, 黄智翔, 等. 动物生物安全实验室生物废弃物安全处置管理与探索[J]. 实验技术与管理, 2020, 37(8):280-283, 288. DOI:10.16791/j.cnki.sjg.2020.08.062 .
24	CENTERS FOR DISEASE CONTROL AND PREVENTION. CDC report on the potential exposure to Anthrax[J]. Int Microbiol, 2014, 17(2):119-127. DOI:10.2436/20.1501. 01.214 .
25	GIBBS E P J. The evolution of One Health: a decade of progress and challenges for the future[J]. Vet Rec, 2014, 174(4):85-91. DOI:10.1136/vr.g143 .

疾病病原体	病原体类型	人兽共患病	可能携带病原体的动物
肝毛细线虫	寄生虫	是	野生啮齿类动物
棘球绦虫	寄生虫	是	野生啮齿类动物
脑胞内原虫	寄生虫	是	野生啮齿类动物
粗带绦虫	寄生虫	是	普通田鼠
带状绦虫	寄生虫	是	普通田鼠
弓形虫	寄生虫	是	普通田鼠
虫媒病毒	病毒	是	野生啮齿类动物
脑心肌炎病毒	病毒	是	野生啮齿类动物
汉坦病毒	病毒	是	普通田鼠、家鼠以及其他野生啮齿类动物
淋巴细胞性脉络丛脑膜炎病毒	病毒	是	普通田鼠
正痘病毒	病毒	是	野生啮齿类动物
副痘病毒	病毒	是	野生啮齿类动物
巴尔通体	细菌	是	普通田鼠
螺旋体	细菌	是	岸鼠、普通田鼠
衣原体	细菌	是	多种野生啮齿动物
伯纳特立克次体	细菌	是	岸鼠、普通田鼠
钩端螺旋体	细菌	是	岸鼠、普通田鼠
鸟型副结核分枝杆菌	细菌	是	黄颈田鼠
立克次体	细菌	是	岸鼠、普通田鼠
金黄色葡萄球菌	细菌	是	野生啮齿类动物

疾病病原体	病原体类型	人兽共患病	可能携带病原体的动物
肝毛细线虫	寄生虫	是	野生啮齿类动物
棘球绦虫	寄生虫	是	野生啮齿类动物
脑胞内原虫	寄生虫	是	野生啮齿类动物
粗带绦虫	寄生虫	是	普通田鼠
带状绦虫	寄生虫	是	普通田鼠
弓形虫	寄生虫	是	普通田鼠
虫媒病毒	病毒	是	野生啮齿类动物
脑心肌炎病毒	病毒	是	野生啮齿类动物
汉坦病毒	病毒	是	普通田鼠、家鼠以及其他野生啮齿类动物
淋巴细胞性脉络丛脑膜炎病毒	病毒	是	普通田鼠
正痘病毒	病毒	是	野生啮齿类动物
副痘病毒	病毒	是	野生啮齿类动物
巴尔通体	细菌	是	普通田鼠
螺旋体	细菌	是	岸鼠、普通田鼠
衣原体	细菌	是	多种野生啮齿动物
伯纳特立克次体	细菌	是	岸鼠、普通田鼠
钩端螺旋体	细菌	是	岸鼠、普通田鼠
鸟型副结核分枝杆菌	细菌	是	黄颈田鼠
立克次体	细菌	是	岸鼠、普通田鼠
金黄色葡萄球菌	细菌	是	野生啮齿类动物

[1]	Yong ZHAO. Evolution and Prospects of Laboratory Animal Management: A Case Study of Shanghai's Development in the Past Decade [J]. Laboratory Animal and Comparative Medicine, 2023, 43(5): 492-503.
[2]	Lianxiang GUO. Revision of Standards for Microbiological and Parasitological Grades in Laboratory Animals and Its Comparison to Foreign Standards [J]. Laboratory Animal and Comparative Medicine, 2023, 43(4): 339-346.
[3]	Huan GOU, Xinying AN, Yujia TONG, Yan WANG, Shuang YANG. Analysis on the Development Status of Laboratory Animals in Japan [J]. Laboratory Animal and Comparative Medicine, 2023, 43(2): 194-204.
[4]	Chongyang QI, Lin CHEN, Man AI, Hang XU, Housen ZHANG. Safety Management Countermeasures Based on the Status of Laboratory Animal Administrative Licensing in Jiangsu Province [J]. Laboratory Animal and Comparative Medicine, 2023, 43(1): 79-85.
[5]	Xiaying LI, Yusheng WEI, Yonglu TIAN, Wanyong PANG. A Brief Interpretation of CCAC Guide on Humane Endpoints of Animals: 2022 Edition [J]. Laboratory Animal and Comparative Medicine, 2022, 42(6): 566-571.
[6]	Jian WANG, Jin LU, Lingyun TAO, Hongkun FU, Cheng GAO, Baojin WU, Yao LI, Yufeng TAO. Analysis on the Standardized Expression of the Accredited Ability Scope for Hematology, Blood Biochemistry and Urinalysis in Laboratory Animal Clinical Testing [J]. Laboratory Animal and Comparative Medicine, 2022, 42(6): 511-517.
[7]	Huiping LI, Chujun LIANG, Weibo ZHAO. The Current Status of Laboratory Animal License Administration in Guangdong Province [J]. Laboratory Animal and Comparative Medicine, 2022, 42(6): 577-582.
[8]	Xiuqing ZHENG, Zhiqiang SHAO, Jing SONG, Yuehua ZHANG, Jinhua SU. Practice of ABSL-2 Laboratory Process Management in Universities： Xiamen University as An Example [J]. Laboratory Animal and Comparative Medicine, 2022, 42(4): 364-370.
[9]	Shibo SU, Wenjie MA, Wei WANG, Huijuan GUO, He ZHANG, Zi WANG, Lei KANG, Shouqi LIU, Huairan LIU, Changde SI, Chuntian GOU, Wei ZHANG, Hongyan CHEN. Performance Test and Evaluation of Self-developed Biosafety Cage Changing Workbench [J]. Laboratory Animal and Comparative Medicine, 2022, 42(2): 102-110.
[10]	Wenjie MA, Wei ZHANG, Wei WANG, Shibo SU, Huijuan GUO, He ZHANG, Zi WANG, Huairan LIU, Hongyan CHEN. Performance Analysis of Class Ⅱ Biosafety Cabinetry in Veterinary-related Research Institutes [J]. Laboratory Animal and Comparative Medicine, 2022, 42(2): 111-116.
[11]	Wei ZHANG, Bin TANG, Yong ZHAO, Zhini ZHANG, Xinyan DENG. Tracking and Monitoring on Application of Micro-electrolyzed Sterile Water System in Laboratory Animal Barrier Facilities [J]. Laboratory Animal and Comparative Medicine, 2022, 42(2): 117-121.
[12]	Yong LI, Liang ZHOU, Yong HE, Zhifeng YAN, Haijiang WANG. Preliminary Exploration of Information Supervison System for Harmless Disposal of Laboratory Animal Carcasses and Related Wastes [J]. Laboratory Animal and Comparative Medicine, 2022, 42(2): 122-126.
[13]	Lixiang CHEN, Boyin QIN, Hua YANG, Chunhua XU, Xiuhua PENG, Shun LI, Xiaohui ZHOU. Application Prospect of Identification and Traceability Technology of Laboratory Animals in Biosafety Laboratory [J]. Laboratory Animal and Comparative Medicine, 2022, 42(2): 89-94.
[14]	CHEN Xiaojuan, LI Wei, WANG Lie. Exploration on Informatization of Laboratory Animal Management Process in Colleges and Universities: The Case of Zhejiang University [J]. Laboratory Animal and Comparative Medicine, 2021, 41(6): 554-558.
[15]	LUO Yinzhu, Min Fangui, WANG Jing, HE Lifang, PAN Jinchun. Prevention and Control of Pathogenic Microorganisms for the Management and Biosafety Control in Laboratory Animal Facilities [J]. Laboratory Animal and Comparative Medicine, 2021, 41(5): 443-449.