乙二胺四乙酸驱镉对慢性镉中毒大鼠、小鼠的肾毒性

doi:10.3969/j.issn.1674-5817.2015.01.012

实验动物与比较医学 ›› 2015, Vol. 35 ›› Issue (1): 56-60.DOI: 10.3969/j.issn.1674-5817.2015.01.012

乙二胺四乙酸驱镉对慢性镉中毒大鼠、小鼠的肾毒性

李光先^1,2, 钟志勇², 陈嘉兴^1,2, 余日安¹, 唐小江²

1.广东药学院公共卫生学院, 广州 510310;
2.广东省医学实验动物中心, 南海 528248

收稿日期:2014-07-17 出版日期:2015-02-25 发布日期:2015-02-25
作者简介:李光先(1987-), 女, 硕士研究生, 主要研究方向:环境与职业毒理学, E-mail:lig_xian@163.com
基金资助:
广东省重大科技专项(2012A080201011), 佛山市院市合作项目(2012A04)

Nephrotoxicity of EDTA Utilized to Treat Chronic Cadmium Poisoning in Rats and Mice

LI Guang-xian^1,2, ZHONG Zhi-yong², CHEN Jia-xing^1,2, YU Ri-an¹, TANG Xiao-jiang²

1. School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China;
2. Guangdong Medical Laboratory Animal Center, Nanhai 528248, China

Received:2014-07-17 Online:2015-02-25 Published:2015-02-25

摘要/Abstract

摘要： 目的探讨乙二胺四乙酸(EDTA)治疗慢性镉中毒动物模型毒副作用。方法将雄性SD大鼠、雄性NIH小鼠分别随机分为空白对照组和模型组。模型组腹腔注射CdCl₂+ β-巯基乙醇(ME)的混合液,每日一次,连续5 d,末次染毒50 d及30 d后分别随机分为模型对照组和EDTA治疗组。空白对照组和模型对照组腹腔注射生理盐水; EDTA治疗组按体质量腹腔注射EDTA溶液,实验结束后均采集动物的尿液、血液肾脏及睾丸组织,检测体内Cd和组织病理检查。结果实验结束时,EDTA组大鼠、小鼠的肾脏Cd、尿Cd和血Cd含量明显高于模型对照组(P<0.05),并见肾小管蛋白质沉淀。结论 EDTA会引起体内的镉向肾脏富集,不宜用于慢性镉中毒驱镉。

关键词: 慢性镉中毒, 二胺四乙酸(EDTA), 肾毒性

Abstract: Objective To investigate the side effects of (EDTA) utilized to treat chronic Cadmium poisoning in animals. Methods Male SD rats and NIH mice were randomly divided to the control group and model group respectively. The model group animals were daily given CdCl₂+ 2-Mercaptoethanol(ME, ip) for 5 days. 50 days or 30 days later, the rats or mice were randomly divided into model control group and EDTA treatment group respectively. The control group and model control group were injected with saline, and the EDTA group animals were treated with EDTA solution .At the end of the experiment, the urine, blood, kidneys and testis were collected for detecting the Cd concentration and pathologic changes. Results Compared with the model control group, in the EDTA treated groups (both in rats and mice), the Cd levels of kidney, urine and blood all increased significantly (P<0.05); protein precipitation could be detected in kidney tubules. Conclusion The EDTA could promote the accumulation of cadmium in the kidney. EDTA is not recommended to treat chronic cadmium poisoning patients.

Key words: Chronic cadmium poisoning, EDTA, Nephrotoxicity

中图分类号:

Q95-33

李光先, 钟志勇, 陈嘉兴, 余日安, 唐小江. 乙二胺四乙酸驱镉对慢性镉中毒大鼠、小鼠的肾毒性[J]. 实验动物与比较医学, 2015, 35(1): 56-60.

LI Guang-xian, ZHONG Zhi-yong, CHEN Jia-xing, YU Ri-an, TANG Xiao-jiang. Nephrotoxicity of EDTA Utilized to Treat Chronic Cadmium Poisoning in Rats and Mice[J]. Laboratory Animal and Comparative Medicine, 2015, 35(1): 56-60.

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乙二胺四乙酸驱镉对慢性镉中毒大鼠、小鼠的肾毒性

Nephrotoxicity of EDTA Utilized to Treat Chronic Cadmium Poisoning in Rats and Mice

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