慢性缺O2高CO2性大鼠小脑水肿及姜黄素的保护作用

doi:10.3969/j.issn.1674-5817.2013.02.005

实验动物与比较医学 ›› 2013, Vol. 33 ›› Issue (2): 106-111.DOI: 10.3969/j.issn.1674-5817.2013.02.005

慢性缺O₂高CO₂性大鼠小脑水肿及姜黄素的保护作用

毛香岚¹, 赵翔¹, 李泽杰¹, 蔡鸿勇¹, 张涛¹, 叶光华², 喻林升²

1.温州医学院基础医学院,温州325000;
2.温州医学院法医学系, 温州 325035

收稿日期:2012-07-19 出版日期:2013-04-25 发布日期:2013-04-25
作者简介:毛香岚(1990-), 女, 本科在读, 温州医学院基础医学院09法医, E-mail: 270295372@qq.com
基金资助:
温州医学院学生科研立项重点资助课题(wyx201201005)

Chronic Hypoxia- high Carbon Dioxide -induced Cerebellum Edema and Protective Effect of Curcumin in Rat

MAO Xiang-lan, ZHAO Xiang, LI Ze-jie, ZHANG Tao, CAI Hong-yong, YE Guang-hua, YU Lin-sheng

College of Basic Medicine, Wenzhou Medical College, Wenzhou 325000, China

Received:2012-07-19 Online:2013-04-25 Published:2013-04-25

摘要/Abstract

摘要： 目的探讨缺O₂高CO₂对大鼠小脑组织的损伤, 并阐明姜黄素对其的保护作用。方法成年SD大鼠15只随机分为3组: 对照组、实验组及姜黄素组。实验组及姜黄素组于常压低O₂高CO₂舱内建立低O₂高CO₂大鼠脑模型。用HE染色、免疫组化及电镜观察缺O₂高CO₂情况下大鼠小脑细胞水肿程度差异; 实时荧光PCR检测水通道蛋白4(AQP4)mRNA在实验组和对照组中的表达差异。结果实验组大鼠小脑细胞水肿程度明显,AQP4基因的表达相对较高; 姜黄素组大鼠小脑细胞水肿程度较轻,AQP4基因的表达明显减少。结论在缺O₂高CO₂的环境中大鼠小脑组织出现水肿,和AQP4基因表达有关,姜黄素对该情况有保护作用。

关键词: 慢性缺O₂高CO₂, AQP4, 姜黄素, 实时荧光PCR

Abstract: Objective To explore expression of AQP4 and edema in hypoxia- high carbon dioxide rat cerebellum edema model and the protective effects of curcumin. Methods Fifty-five adult male SD rats were randomly divided into 3 groups: the control group, the experimental group and curcumin treated group. The experimental group and curcumin group were housed in atmospheric hypoxia- high carbon dioxide environment to induce hypoxia-high carbon dioxide brain edema model. The difference of the hypoxia-high carbon dioxide of rat cerebellar cells edema degree were observed under electron microscopic after HE staining and immunohistochemistry. The expression level of AQP4mRNA in the experimental group and the control group were detected using Real time fluorescence PCR. The results showed that cerebellar cells edema was more significant and AQP4 gene expression was relatively higher in the experimental group rat than curcumin group. Conclusion Rat cerebellar edema can be developed in hypoxia-high carbon dioxide environment, and it may be related to the expression of AQP4 gene, Curcumin has protective effect on such edema.

Key words: Hypoxia-high carbon dioxide, AQP4, Real time fluorescence PCR, Curcumin

中图分类号:

Q95-33

毛香岚, 赵翔, 李泽杰, 蔡鸿勇, 张涛, 叶光华, 喻林升. 慢性缺O₂高CO₂性大鼠小脑水肿及姜黄素的保护作用[J]. 实验动物与比较医学, 2013, 33(2): 106-111.

MAO Xiang-lan, ZHAO Xiang, LI Ze-jie, ZHANG Tao, CAI Hong-yong, YE Guang-hua, YU Lin-sheng. Chronic Hypoxia- high Carbon Dioxide -induced Cerebellum Edema and Protective Effect of Curcumin in Rat[J]. Laboratory Animal and Comparative Medicine, 2013, 33(2): 106-111.

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慢性缺O₂高CO₂性大鼠小脑水肿及姜黄素的保护作用

Chronic Hypoxia- high Carbon Dioxide -induced Cerebellum Edema and Protective Effect of Curcumin in Rat

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