地塞米松对急性坏死性胰腺炎大鼠模型血液TNF-α、IL-10和GR的影响

›› 2008, Vol. 28 ›› Issue (4): 230-233.

地塞米松对急性坏死性胰腺炎大鼠模型血液TNF-α、IL-10和GR的影响

温州医学院附属第一医院 1.普外科，温州 325000；2.检验科，温州 325000

收稿日期:2008-03-24 出版日期:2008-04-30 发布日期:2008-04-30

Influence of Dexamethasone on Blood TNF-α,IL-10,GR in Rats Models of Acute Necrotizing Pancreatitis

1. Department of General Surgery, 2. Department of Laboratory,The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China

Received:2008-03-24 Online:2008-04-30 Published:2008-04-30

摘要/Abstract

摘要： 目的探讨急性坏死性胰腺炎（ANP）大鼠模型血液TNF-α、IL-10和糖皮质激素受体（GK）的变化规律及地塞米松（DEX）干预对上述指标的影响.方法 48只雄性SD大鼠随机分为ANP组（A组，n =16）、DEX治疗组（D组，n =16）和假手术组（S组，n =16）.除S组大鼠外，各组均通过逆行胰胆管注射5%牛磺胆酸钠制作.ANP模型；D组大鼠术后肌注地塞米松干预.制模后8、12 h分别处死各组一半大鼠，分别观察大鼠胰腺病理改变、血清淀粉酶、TNF-α、IL-10水平和全血有核细胞GR的mRNA表达等指标.结果 ①A组大鼠表现为典型ANP病理改变，包括间质水肿、炎细胞浸润、出血和胰腺坏死；D组大鼠胰腺的炎症反应相对较轻.②A组血清TNF-α、IL-10显著高于S组（P＜0.01），D组血清TNP低于A组而IL-10高于A组（P＜0.01）.③在术后8 h和12h，D组血GRmRNA表达显著低于S组（分别P＜0.05，P＜0.01）.结论 TNF-α、IL-10以及GR的改变与ANP的炎症反应存在相关性，外源性DEX可能通过影响这些因素达到缓解ANP的作用.

关键词: 地塞米松, 急性坏死性胰腺炎, INF-α, IL-10, 糖皮质激素受体

Abstract: Objective To investigate the changes of TNF-a, IL-10, glucocorticoid receptor (GR) in Rats with acute necrotizing pancreatitis (ANP) and the influence of Dexamethasone(DEX) on these parameters in ANP rats. Methods Fourty-eight male Sprague-Dawley (SD) rats were randomly divided into acute-necrotizing-pancreatitis group (group A,n=16)，DEX-treated group (group D, n=16) and sham-operation group(group S，n=16). ANP model was induced by intergraded injection of 5% sodium tanrocholate to biliopancreatic duct in the rats except for group S, and the rats in group D were intramuscularly given dexamethasone after operation. The change of pancreatic pathology, the serum AMY, TNF-a and IL-10 level, and the blood mRNA expression of GR were examined. Results ①Typical ANP pathological changes were found in group A, including i.e. interstitial edema, infiltration of inflammatory cells, hemorrhage and parenchyma necrosis; and the mild inflammation was found in rats of D group which treated by Dexamethasone.② The concentration of serum TNF-a, IL-10 in group A were obviously higher than that in group S (P＜0.01). The concentration of serum TKF-a decreasing and IL-10 increasing in rats were induced by the usage of Dexamethasone (P＜0.01).③ The mRNA expression of blood GR in group D was lower significantly at 8h or 12h after the operation than that in group S (P＜0.01 or P＜0.05). Conclusions The changes of TKF-a, IL-10, GR are involved in the inflammation response of rat ANP, and the dexamethasone can relieve ANP inflammation response by affecting these factors.

Key words: Dexamethasone, Acute necrotizing pancreatitis, TKF-α, IL-10, Glucocorticoid receptor

刘纳新1，刘存丽2，陈周浔1，施红旗1. 地塞米松对急性坏死性胰腺炎大鼠模型血液TNF-α、IL-10和GR的影响[J]. , 2008, 28(4): 230-233.

LIU Na-xin1,LIU Cun-li2,CHEN Zhou-Xun1,SHI Hong-Qi1. Influence of Dexamethasone on Blood TNF-α,IL-10,GR in Rats Models of Acute Necrotizing Pancreatitis[J]. , 2008, 28(4): 230-233.

[1]	许冰馨, 范凯健, 王婷玉, 陈慧瑾. 地塞米松对胶原诱导性关节炎大鼠模型软骨退化的改善作用[J]. 实验动物与比较医学, 2022, 42(5): 416-422.
[2]	李海燕，黎家敏，李靖潇，王新兴，代解杰，孙晓梅. 高糖高脂饲料联合地塞米松诱发树鼩血糖、血脂异常[J]. , 2010, 30(3): 197-200.
[3]	刘纳新1，管敏强2，陈通克2，赵惠玲2，陈锡文2. 两种大鼠急性坏死性胰腺炎模型制作方法的改良和比较[J]. , 2008, 28(2): 110-113.
[4]	崔世涛, 朱洪生. 地塞米松对大鼠离体心脏再灌注损伤保护机制的研究[J]. , 1998, 18(2): 69-72.