FMMU白化豚鼠类鼻疽病模型的建立

›› 2008, Vol. 28 ›› Issue (3): 167-170.

FMMU白化豚鼠类鼻疽病模型的建立

1.南方医科大学实验动物中心暨比较医学研究所，广州 510515；2.南方医科大学珠江医院病理科，广州 510282

收稿日期:2008-04-10 出版日期:2008-03-31 发布日期:2008-03-31

Establishment of Melioidosis Model with FMMU Albino Guinea Pig

1. Laboratory Animal Center & Institute of Comparative Medicine, Southern Medical University, Guangzhou 510515, China; 2. Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China

Received:2008-04-10 Online:2008-03-31 Published:2008-03-31

摘要/Abstract

摘要： 目的建立FMMU白化豚鼠类鼻疽动物模型。方法类鼻疽杆菌腹腔接种FMMU白化豚鼠建立感染模型，观察模型动物外观、血液含菌量、死亡率以及主要脏器病理学变化，电子显微镜观察豚鼠白细胞杀灭类鼻疽杆茵作用。结果感染豚鼠出现疾病征象，造模后72 h内血液含菌量达到约1 4000CFLJ/ml，造模后96h死亡率70%，光镜观察发现主要在脾，淋巴结、肝等实质器官中存在典型的化脓灶，电镜观察表明豚鼠白细胞具有杀灭类鼻疽杆菌作用。结论成功建立FMMU白化豚鼠类鼻疽杆菌感染模型，可作为人类的急性类鼻疽病动物模型用于相关研究。

关键词: 类鼻疽, 类鼻疽杆菌, FMMU白化豚鼠

Abstract: Objective To establish melioidosis model of FMMU albino guinea pig. Method FMMU albino guinea pig infection model were made by intraperitoneal inoculation of Burkholderia pseudomallei. The clinical manifestations, the amount of bacteria in blood，mortality rate and histopathologic changes in major parenchymatous organs were observed, the killing effect of polymorphonuclear leukocyte on Burkholderia pseudomallei was observed by electron micro scope (EM). Result The characteristic sign of the melioidosis appeared after inoculation, the amount of bacteria in blood was 1 4000 CFU/ml at 72 h post inoculation，mortality rate was 70% at 96 h post inoculation. Under light microscope there were typical suppuration focus in parenchymatous organ. Polymorphonuclear leukocyte could kill the Burkholderia pseudomallei when observed under EM. Conclusion Burkholderia pseudomallei infection model of FMMU albino guinea pig was established successfully and may be used for research on acute Melioidosis in human.

Key words: Melioidosis, Burkholderia pseudomallei, FMMU albino guinea pig

王万山1，刘真喜2，张嘉宁1，吴清洪1，顾为望1. FMMU白化豚鼠类鼻疽病模型的建立[J]. , 2008, 28(3): 167-170.

WANG Wan-shan1,LIU Zhen-xi2,ZHANG Jia-ning1,WU Qing-hong1,GU Wei-wang1. Establishment of Melioidosis Model with FMMU Albino Guinea Pig[J]. , 2008, 28(3): 167-170.

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