›› 2008, Vol. 28 ›› Issue (3): 155-159.

• 论文 • 上一篇    下一篇

西藏小型猪MSTN基因5'调控区的酶切多态性分析

  

  1. 1.南方医科大学实验动物中心暨比较医学研究所,广州 510515;2.中国人民解放军421医院,广州 510318
  • 收稿日期:2008-04-10 出版日期:2008-03-31 发布日期:2008-03-31
  • 基金资助:
    广东省农业攻关计划(2006B20201053),广东省科研条件建设项目(2006B60101059)

The Study of Tibet Mini-pigs Myostatin Gene in 5' Controllable Region by PCR-RFLP

  1. 1. Laboratory Animal Centre & Institute of Comparative Medcine, Southern Medical University, Guangzhou 510515, China; 2. The 421stHospital ofPLA, Guangzhou 510318, China
  • Received:2008-04-10 Online:2008-03-31 Published:2008-03-31

摘要: 目的 对西藏小型猪MSTN基因进行PCR-RFLP基因多态性分析,并对五指山小型猪、贵州香猪和巴马小型猪进行MSTN基因多态性的比较研究。方法 以42头西藏小型猪、17头五指山小型猪、7头巴马小型猪和22头贵州香猪作为研究对象,对猪MSTN基因5' 调控区进行了PCR-RFLP多态性研究。结果 1、不同猪种之间的基因型频率和等位基因频率有显著性差异(P=0.000),西藏小型猪的MSTN基因表现出3种基因型TT(9.52%)、AA(61.90%)、TA(28.57%),T和A的频率分别为23.81%和76.19%;五指山小型猪的MSTN基因表现出2种基因型TT(82.35%)、AA(17.65%),T和A的频率分别为82.35%和17.65%;贵州香猪的MSTN基因表现出2种基因型TT(81.82%)、TA(18.18%),T和A的频率分别为90.91%和9.09%;巴马小型猪的MSTN基因表现出2种基因型TT(71.43%)和TA(28.57%),T和A的频率为85.71%和14.29%。2、不同MSTN基因型的西藏小型猪的初生重(P=0.761)和离乳重(P=0.319)无显著性差异。结论 西藏小型猪、五指山小型猪、贵州香猪和巴马小型猪的MSTN基因5'调控区存在多态性,但暂不能把MSTN基因做为西藏小型猪繁殖性能的分子标记。

关键词: 西藏小型猪, 五指山小型猪, 巴马小型猪, 贵州香猪, MSTN基因, PCR-RFLP

Abstract: Objectives To analyze the MSTN gene of Tibet mini-pigs,WU Zhi-shan mini-pigs,Bama mini-pigs and Guizhou mini-pigs.Methods Taking 42 Tibet mini-pigs,17 Wu Zhi-shan mini-pigs,7 Bama mini-pigs and 22 Guizhou mini-pigs as samples,PCR-RFLP polymorphism analysis on the controllable region of the MSTN gene 5’of pigs were carried out.42 Tibet mini-pigs were used to analyze the relation between the RFLP polymorphism of MSTN gene and some breeding characters(weight at birth(WB)and weaning weight(WW)).Results(l)There is significant difference in genetype frequencies and allele frequencies between different stocks of pigs(P=0.000).Tibet mini-pigs have three MSTN genetypes TT(9.52%)、AA(61.90%)、TA(28.57%)、and the frequencies of alleles T and A is 23.81%and 76.19%respectively;Wu Zhi-shan mini-pigs have two genetypes TT(82.35%)、AA(17.65%)、and the frequencies of alleles T and A is 82.35%and 17.65%respectively;Guizhou minipigs have two genetypes TT(81.82%)、TA(1 8.18%)、and the frequencies of alleles T and A is 90.91%and 9.09%respectively;Bama mini-pigs have two genetypes TT(71.43%)、TA(28.57%)、and the frequencies of alleles T and A is 85.71%and 14.29%respectively.(2)The difference of WB(P=0.761)and WW(P

Key words: Tibet mini-pigs, WU Zhi-shan mini-pig, Bama mini-pigs, Guizhou mini-pigs;MSTN gene, PCR-RFLP