关键词: α-1, 3-半乳糖苷转移酶(α-GT), 基因沉默, 小鼠, 小干扰RNA, 转基因

Abstract: Objective To product α-l，3-GT transgenic mice animal model for the study of xenograft. Method The α-l，3-GT genes was introduced into fertilized mice eggs of one-cell stage by microinjection. Result Eight hundred and fifty-eight fertilized eggs were introduced with α-l，3-GT gene. Of them, 312 fertilized mice eggs of one-cell stage were transplanted into 11 pseudpregnant mice, 6 of them were pregnanted (54.5%) and were aborted; 541 fertilized mice eggs of two-cell stage were transplanted into 23 pseudpregnant mice，18 of them were pregnanted (78.3%) and 11 pregnant females were aborted, 14 dead offspring were bom from three pregnant females. None were proved to be gene integration positively; 21 dead offspring were bom from four pregnant females of days or so, 7 of them were proved to be gene integration positively. Conclusion Transgenic mice have been obtained through microinjection of α-1, 3-GT genes, and further study needed to product the live ot-l，3-GT transgenic mice offspring.

Key words: α-1,3-GT, Gene silence, Mouse, RNA interference, Transgene