大鼠松果体N-乙酰基转移酶基因的内源性昼夜表达规律

实验动物与比较医学 ›› 2007, Vol. 27 ›› Issue (4): 215-218.

• 论文 •

大鼠松果体N-乙酰基转移酶基因的内源性昼夜表达规律

苏州大学 1.医学院基础医学系生理学教研室；2.放射医学与公共卫生学院卫生毒理学教研室，苏州 215123

基金资助:
国家自然科学基金（30170295），苏州大学医学发展基金（EE134031）及青年教师研究基金（Q3134044）项目资助

Endogenous Circadian Expression of the Arylalkylamine N-acetyltransferase Gene in the Pineal Gland of Rat

1. Dept, of Physiology, Medical School; 2. Dept, of Toxicology, School of Radiation Medicine & Public Health, Soochow University, Suzhou 215123, China

摘要/Abstract

摘要： 目的探讨大鼠松果体N-乙酰基转移酶（NAT）基因的内源性昼夜节律表达.方法 SD大鼠在持续黑暗（DD）光制下饲养8周后，在一昼夜内每隔4h采集一组松果体组织，提取总RNA，进行竞争性定量RT-PCR，测定不同昼夜时点（CT）样品中NAT基因mRNA的相对表达量，用余弦函数软件获取节律参数，并经振幅F检验分析是否存在昼夜节律.结果松果体NAT基因mRNA的表达呈现明显的内源性节律振荡（P＜0.05），峰值（mRNA水平为1.42±0.11）和谷值（mRNA水平为0.56±0.18）分别位于CT17和CT5，峰值相位-260.85±9.45，振幅0.44±0.09，中值0.99±0.12.结论大鼠松果体NAT基因存在明显的内源性昼夜节律表达，其转录水平在主观黑夜高于主观白天.

Abstract: Objective To investigate the endogenous circadian expression of the Arylalkylamine N-acetyltransferase（NAT）gene in the rat pineal gland.Methods 36 SD rats under constant darkness（DD）for eight weeks were sampled for the pineal gland once every 4 hours in a circadian day.The total RNA was extracted from the samples and the semi quantitative reverse transcription polymerase chain reaction（RT-PCR）was used to determine the temporal changes in mRNA level of the NAT gene during different circadian times（CT）.The data parameters were obtained and analysed by both the cosine function software and the amplitude F test to reveal the circadian rhythm.Results The NAT mRNA level in the pineal gland showed a significantly circadian oscillation（〈i>P

. Endogenous Circadian Expression of the Arylalkylamine N-acetyltransferase Gene in the Pineal Gland of Rat[J]. Laboratory Animal and Comparative Medicine, 2007, 27(4): 215-218.

大鼠松果体N-乙酰基转移酶基因的内源性昼夜表达规律

Endogenous Circadian Expression of the Arylalkylamine N-acetyltransferase Gene in the Pineal Gland of Rat

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