IVF-20培养液用于四个品系大鼠体外受精的初步研究

实验动物与比较医学 ›› 2007, Vol. 27 ›› Issue (2): 99-103.

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IVF-20培养液用于四个品系大鼠体外受精的初步研究

1.南京农业大学动物医学院，南京 210095；2.中国科学院上海实验动物中心，上海，201615；3.扬州大学比较医学中心，扬州 225009；4.沈阳农业大学畜牧兽医学院，沈阳 110161

基金资助:
上海市科技发展基金（0549090007），国家科技基础条件平台项目（2003DEA3N033）

Studies on In Vitro Fertilization by Using IVF-20 Media in Four Strains of Rat

1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;2. Shanghai Laboratory Animal Center, Chinese Academy of Sciences, Shanghai 201615，China;3. Comparative Medicine Center, Yangzhou University, Yangzhou 25009, China;4. College of Animal Science and Veterinary, Shenyang Agricultural University, Shenyang 110161, China

摘要/Abstract

摘要： 目的系大鼠体外受精后的2细胞发育率分别可达83.2%、72.6%、87.8%和71.6%.体外受精的2细胞胚胎能够在体外进一步发育，SD大鼠囊胚发育率为16.7%，与体内受精胚胎在体外培养的囊胚发育率（24.5%）差异不显著.结论用于人体外受精的IVF-20培养液同样适合于实验大鼠精子的体外获能和受精，可以在多种品系获得较高的2细胞发育率，并且所得2细胞能够在体外发育到囊胚.

Abstract: Objective To investigate the feasibility of IVF-20 medium on the in vitro fertilization (IVF) in Rats. Methods Using IVF-20 as capacitation and fertilization medium, the IVF in four strains, including SD, Wistar, GK and F344 were conducted. Besides, the embryos from SD rat fertilized both in vitro and in vivo were cultured using mRlEMC medium to test their viability. Result The fertilization rates in SD, Wistar, GK and F344 were 83.2%、72.6%、87.8 % and 71.6% respectively. The rate of embiyos developed to blastula was 16.7%, having comparative viability of embryos fertilized in vivo (24.5%). Conclusion The IVF-20 medium, which had been used in vitro fertilization of human, is also a suitable medium for the capacitation and fertilization in rats.

. Studies on In Vitro Fertilization by Using IVF-20 Media in Four Strains of Rat[J]. Laboratory Animal and Comparative Medicine, 2007, 27(2): 99-103.

IVF-20培养液用于四个品系大鼠体外受精的初步研究

Studies on In Vitro Fertilization by Using IVF-20 Media in Four Strains of Rat

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