血管抑素基因联合氟尿嘧啶化疗对荷瘤裸小鼠抗恶病质效应的影响

›› 2007, Vol. 27 ›› Issue (2): 104-106.

血管抑素基因联合氟尿嘧啶化疗对荷瘤裸小鼠抗恶病质效应的影响

1.复旦大学实验动物科学部，上海 20032；2.申科院上海实验动物中心，上海 201615；3.上海市华东医院普外科，上海 200040

收稿日期:2006-07-19 出版日期:2007-02-28 发布日期:2007-02-28
基金资助:
上海市自然科学基金（04Z14045）资助

Effect of Combining Angiostatin Gene Therapy and 5-FU Chemotherapy on Cachexia in Nude Mice Bearing with Colorectal Carcinoma in Abdominal Cavity

Department of Urology, the First Hospital Affiliated to Suzshou University, Suzhou 215006，China

Received:2006-07-19 Online:2007-02-28 Published:2007-02-28

摘要/Abstract

摘要： 目的观察血管抑素基因联合氟尿嘧啶腹腔化疗对抗腹腔荷瘤裸小鼠的抗恶病质效应.方法 RT-PCR克隆血管抑素基因，建立重组腺病毒载体.建立人结肠癌LoVo细胞腹腔种植的模型，给予血管抑素基因联合氟尿嘧啶腹腔化疗.观察荷瘤裸小鼠的体重和摄食量的改变及血清TNF-α和IL-1水平的变化.结果血管抑素基因联合氟尿嘧啶腹腔化疗组裸小鼠的体重、摄食量、精神和活动情况明显好于对照组；治疗组的血清TNF-α和IL-1水平和肿瘤微血管密度（MVD）明显低于其他各组.结论血管抑素的基因治疗与传统的腹腔化疗联合应用，可明显的改善荷瘤裸小鼠的恶病质症状，增强其抗恶病质的效应.

关键词: 血管抑素, 大肠癌, 氟尿嘧啶, 基因治疗, 恶病质

Abstract: Objective To induce renal transplantation tolerance by CTLA4-Ig adenovirus gene therapy in allogeneic rats and study the relative mechanisms of transplantation tolerance.Methods The kidney of SD rats were transfected with CTLA4-Ig gene-recombined adenovirus.After the orthotopic renal transplantation between SD and Wistar rats was conducted,all the rats were divided into 3 groups:group I was the control;rats in group II were perfused with empty EGFP and there in group Ⅲ with CTLA4-Ig adenovirus. The allograft survival time and the allograft funtion between the three groups were compared. Result The survival time was significantly longer in rat of group Ⅲ than in other 2 groups(31.3 ±6.8 days vs 8.2 士 1.2 days and 8.0 士 1.6 days). The serum creatinine of transfection group at 7th and 14th postoperative days was lower than that of controls at 7th postoperative day. Conclusion Local transfection of CTLA4-Ig gene can augment the tolerance and prolong the survival time of renal allograft significantly.

Key words: Gene expression, Transplantation, Kidney

乔伟伟1，顾坚忠2，杜卫东3. 血管抑素基因联合氟尿嘧啶化疗对荷瘤裸小鼠抗恶病质效应的影响[J]. , 2007, 27(2): 104-106.

QIAO Wei-wei1,GU Jian-zhong2,DU Wei-dong3. Effect of Combining Angiostatin Gene Therapy and 5-FU Chemotherapy on Cachexia in Nude Mice Bearing with Colorectal Carcinoma in Abdominal Cavity[J]. , 2007, 27(2): 104-106.

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