›› 2006, Vol. 26 ›› Issue (3): 165-168.

所属专题: 实验动物资源开发与利用

• 论文 • 上一篇    下一篇

普通级Z:ZCLA长爪沙鼠在11个生化基因位点多态性的初步研究

  

  1. 浙江省医学科学院,浙江省实验动物中心,杭州 310013
  • 出版日期:2006-09-25 发布日期:2006-09-25
  • 基金资助:

    浙江省医药卫生基金(2001ZX014)

Elementary study on Genetic Diversity of Biochemical Gene Markers of Z:ZCLA Mongolian Gerbil

  1. Zhejiang Academy of Medical Sciences, Zhejiang Centre of Laboratory Animals, Hangzhou 310013, China
  • Online:2006-09-25 Published:2006-09-25

摘要: 目的 探讨Z:ZCLA长爪沙鼠生化基因位点的多态性。方法 用Gpd-1、Es-1、Es-2、Es-3、Es-4、Es-6、Es-8、Es-9、Es-10、Akp-1、Idh-1、Mod-1、Ce-2、Pgm-1、Gpi-1、Car-2、Hbb、Trf、Cs-1等19个生化标记位点的乙酸纤维素膜电泳技术研究了普通级Z:ZCLA长爪沙鼠的遗传多态性。结果 Z:ZCLA长爪沙鼠在Es-1、Car-2、Hbb、Gpi-1、Cs-1、Ce-2、Id-1、Mod-1上呈单态性,在Es-2,Es-3,Es-4,Es-6,Es-8,Es-9,Es-10,Gpd-1,Pgm-1,Trf, Akp-1个位点上呈现多态性,等位基因从2~4个不等,平均等位基因数3.0。结论 目前本群遗传多样性水平处于中度多态。

关键词: Z:ZCLA长爪沙鼠, 生化基因位点, 遗传多态性

Abstract: Objective To probe into the genetic diversity of the conventional closed colony of Z:ZCLA Mongolian gerbil. Methods The polymorphism of the conventional closed colony of Z:ZCLA Mongolian gerbil(sample content=100 individual) was studied by 19 biochemical gene markers and recorded their characters. Results Among the 19 biochemical loci, The locus Es-1, CarL-2, Hbb, Gpi-1,Cs-1, Ce-2,Id-1,Mod-1 had only one allele, while the locus Es-2, Es-3, Es-4, Es-6, Es-8, Es-9, Es-10, Gpd, Pgm, Trf and Akp-1 had two to four alleles, respectively. Average allele number was recorded as 3.0. Conclusion The conventional closed colony of Z: ZCLA Mongolian gerbil has the medium genetic diversity.

Key words: Z:ZCLA Mongolian gerbil, Biochemical gene Markers, Genetic Diversity