新型禽用抗病毒制剂的研制与应用

实验动物与比较医学 ›› 2006, Vol. 26 ›› Issue (3): 155-159.

• 论文 •

新型禽用抗病毒制剂的研制与应用

1.中国药科大学,南京 210009；2.南京哈耶科技有限公司,南京 210014

Development and Application of A Novel Antiviral Biologic Products for Poultry

1.China Pharmaceutical University,Nanjing 210009,China；2.Nanjing Harya Technology Co.,Ltd., Nanjing 210014,China

摘要/Abstract

摘要： 目的以重组禽干扰素α（IFNα）为基础，针对禽病毒性疾病的预防和治疗，研制禽用抗病毒制剂。方法将禽干扰素α、胸腺肽α等进行基因重组改造，结合先进的发酵工艺，在E.coli和Picha pastoris高效表达系统中分别表达，再分离、纯化。结果不同载体和不同菌株表达的IFN-THY融合蛋白的IFN活性测定结果有一定的差异.96 hIFN活力在0.13×10⁴～8.12×10⁴IU/ml，110h为1.22×10⁵～3.27×10⁶IU/ml。结论 48～110h不同样品均有明显的THY活性。

Abstract: Objective Based on recombination IFNA6 （Chicken interferon alpha）, anti-viral biologic products were developed, aiming at prevention and treatment of poultry viral disease. Method Chicken interferon alpha（IFN） and thymosin alpha（THY） were recombined and expressed in E.coli and Picha pas tor is. Results There were different IFN biological activity from fusion protein of Chicken interferon and thymosin （IFN-THY） expressing in different vectors and different strains, 0.13 X 10⁴-8.12 X 10⁴IU/ml at 96 hours, and 1.22 X 10⁵-3.27 X 10⁶ IU/ml at 110 hours. Conclusion There were significant THY biological activity from different samples at 48-110 hours.

. Development and Application of A Novel Antiviral Biologic Products for Poultry[J]. Laboratory Animal and Comparative Medicine, 2006, 26(3): 155-159.

新型禽用抗病毒制剂的研制与应用

Development and Application of A Novel Antiviral Biologic Products for Poultry

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章

编辑推荐

Metrics

本文评价