Abstract: Objective To assess the effect of frozen methods and cryoprotections on quality of frozen sperm from Beagle dog. Methods Five male Beagle dogs were chosen for collection of semen and 25 fractions of semen samples were diluted with three kinds of extenders and frozen separately using two methods. Result Average post thaw progressive motility were 51.9% and 53.9，and the percentage of sperms with intact acrosome were 36.4% and 37.6%, respectively. The percentage of sperms with intact acrosome supplemented with protect preservative agent Ⅲ were 40.7 and 41.3 by the frozen-straw method and pellet method, which better than the other agent, but only the percentage of sperms with intact acrosome was significant （P＜0.05）. Conclusion The frozen-straw method was better than the pellet method, but there was no statistical difference. The extender supplemented with vitamin E, B6 and glutamic acid is superior to the other two extenders in the average post thaw progressive motility, the percentages of recovery sperms and the percentage of sperms with intact acrosome, but only the percentage of sperms with intact acrosome was significant （P＜0.05）.