实验动物与比较医学 ›› 2005, Vol. 25 ›› Issue (4): 215-217.

• 论文 •    

联合化疗诱导小鼠在体肿瘤多药耐药模型的建立

  

  1. 1.山东省疾病预防控制中心,济南 250014；2.山东中医药大学附属医院,济南 250011；3.山东省中医药研究院,济南 250014；4.山东省实验动物中心,济南 250015
  • 基金资助:

    山东省自然基金资助项目(编号:Y2002C37)

Establishing Mice Model of Multidurg Resistance of Tumor Induced by Combined-chemotherapy

  1. 1.Shandong Center for Disease Control and Prevention, Jinan 250014, China；2.Teaching Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011，China；3.Shandong Academy of TCM, Jinan 250014, China；4.Shandong Provincial Center of Laboractory Animal Jinan 250015, China

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摘要： 目的 建立小鼠在体肿瘤多药耐药模型。方法 模拟临床以亚于治疗剂量的联合化疗PFC方案，诱导S180腹水型小鼠4周，流式细胞仪荧光检测P170、LRP、TOPOⅡ，建立能客观反应临床肿瘤多药耐药产生机制并适合中药干预肿瘤多药耐药研究的在体细胞模型。结果 化疗诱导后昆明小鼠S180腹水肿瘤细胞P170、LRP的表达率和ROPOⅡ活性明显提高，经传代后其表达稳定。结论 联合化疗诱导建立小鼠在体肿瘤多药耐药模型是可行的。

Abstract: Objective To establish the mice model of multidurg resistance of tumorous expression in ascites with SI80 cell. Methods The mice with SI80 cell in ascites were treated with the method of PFC of repeated-combined-chemotherapy in less than the therapy dose for 4 weeks. The levels of P170 and LRP and TOPOⅡ were detected by Flow Cytometry Analysis. Results The cellular multidurg rdsistance of tumorous expression （P170 and LRP） and the activity of TOPOⅡ of mice were improved obviously and the expression was stable in the cell line.

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