应用RT-PCR技术检测啮齿类动物冠状病毒

›› 2004, Vol. 24 ›› Issue (4): 218-220.

应用RT-PCR技术检测啮齿类动物冠状病毒

广东省实验动物监测所，广州 510260

收稿日期:2004-04-15 出版日期:2004-01-25 发布日期:2013-03-19
基金资助:
广东省防治非典科技攻关项目（实验动物驯养动物溯源专题）

Detection of Rodent Coronavirus by Using Reverse Transcriptase Polymerase Chain Reaction

Guangdong Laboratary Animals Monitoring Institute, Guangzhou 510260 China

Received:2004-04-15 Online:2004-01-25 Published:2013-03-19

摘要/Abstract

摘要： 为了检测啮齿类动物冠状病毒，设计了特异性引物，建立RT-PCR检测方法，并用于检测。在基因文库中查找了啮齿类动物冠状病毒和SARS病毒的基因序列，从N基因片段中，利用计算机软件设计了一对特异性引物，通过确定反应混合物的浓度和反应条件，并建立了一整套从病毒RNA抽提、反转录到PCR反应的快速检测啮齿类动物冠状病毒的方法，扩增出了148bp的目的片段。并已用于啮齿类动物中检测各组织和拭子中的冠状病毒。该方法对啮齿类动物冠状病毒的早期诊断和与SARS病毒区别有重要意义。

关键词: 反转录酶-PCR, 啮齿类动物冠状病毒, SARS冠状病毒

Abstract: In order to detect rodent coronavirus, a pair of PCR primers were designed according to the compared sequences of rodent coronavirus and SARS coronavirus. The primers derived from the specific and conservative N gene，RT-PCR method was established. Result of rodent RT-PCR amplification showed the amplified products of 148bp length； RT-PCR method of rodent coronavirus has been used to detect the samples. The rodent RT-PCR method is important to determine early rodent coronavirus and discriminate SARS coronavirus.

Key words: Reverse Transcriptase polymerase chain reaction, Rodent Coronavirus, SARS Coronavirus

刘忠华, 黄韧, 魏社林, 刘香梅, 陈梅丽. 应用RT-PCR技术检测啮齿类动物冠状病毒[J]. , 2004, 24(4): 218-220.

LIU Zhong-Hua, HUANG Ren, WEI She-Lin, LIU Xiang-Mei, CHEN Mei-Li. Detection of Rodent Coronavirus by Using Reverse Transcriptase Polymerase Chain Reaction[J]. , 2004, 24(4): 218-220.