苏云金杆菌的急性毒性及致敏实验观察

实验动物与比较医学 ›› 2004, Vol. 24 ›› Issue (3): 157-159.

苏云金杆菌的急性毒性及致敏实验观察

江西省劳动卫生职业病防治研究所，南昌 330006

收稿日期:2004-05-31 出版日期:2004-01-25 发布日期:2013-03-19

Acute Toxicity and Dermal Sensitzation of Bacillus thuringiensis Original Drug

Jiangxi Institute of Labour Hygiene and Occupational Medicine,Nanchang 330006, China

Received:2004-05-31 Published:2004-01-25 Online:2013-03-19

摘要/Abstract

摘要： 按照《农药登记毒理学试验方法》（GB15670-1995），对生物农药苏云金杆菌原药进行急性经口、经皮、吸入毒性试验，眼睛刺激试验和致敏试验。该受试物对雄、雌性大鼠急性经口LD50为3830、3160mg/kg；对雄、雌性大鼠急性经皮LD₅₀（4h）＞2150mg/kg；对雄、雌性大鼠急性吸入LC₅₀（2h）＞5000mg/m³；对实验兔急性眼平均刺激指数为0；对豚鼠致敏试验，致敏率为0%。因此，该受试物急性经口属微毒性、急性经皮属低毒性、急性吸入属微毒性，眼睛刺激试验为无刺激性，致敏试验属Ⅰ级弱致敏物。

关键词: 生物农药, 苏云金杆菌原药, 急性毒性实验, 致敏实验

Abstract: According to toxicological test methods of pesticides for registration（GB 15670-1995）,The test of acute toxicity of biopesticide - bacillus thuringiensis, include acute oral toxicity, acute dermal toxicity, acute inhalation toxicity, acute eye irritation test and dermal sensitization were carried out. The acute oral toxicity LD₅₀ of this biopesticide was 3830 mg/kg in male and 3160 mg/kg in female, acute dermal toxicity LD₅₀（4h）>2 150 mg/kg, LC₅₀（2h）>5 000 mg/m³, there is no irritation to rabbit eye, dermal sensitization：rate of sensitization is zero.So the acute oral toxicity, acute dermal toxicity and acute inhalation toxicity of bacillus thuringiensis are lower. There is no irritation to rabbit eye, and it belongs to grade I weak sensitizing matter.

Key words: Biopesticide, Bacillus thuringiensis, Acute toxicity test, Dermal sensitization

刘志勇,李启富,周银平,等. 苏云金杆菌的急性毒性及致敏实验观察[J]. 实验动物与比较医学, 2004, 24(3): 157-159.

LIU Zhi-Yong,LI Qi-Fu,ZHOU Yin-Ping,et al. Acute Toxicity and Dermal Sensitzation of Bacillus thuringiensis Original Drug[J]. Laboratory Animal and Comparative Medicine, 2004, 24(3): 157-159.