Abstract: To establish the minipig model with the myocardial infarction or ischimia,and observation marker proving the model. 12 minipigs with body weight 16-22 kilogram were divided into two groups，one was treated with circumflex branch ligation for the myocardial infarction,the other was treated circumflex branch with ameroid constrictor for myocardial ischemia. Both the models underwent exercise and resting myocardial perfusion imaging with single photon emission computed tomography （SPECT） using ^99mTc-MIBI,they also underwent the staining with 2，3-triphenyltetretrazolium chloride in order to distinguish the myocardial infarction or ischemia with isolated heart. The results showed that the left ventricle of the model was observed the defect of ^99mTc-MIBI in the infarction region where the staining of TTC was no uptake. The other model was observed rarefaction of ^99mTc-MIBI in the ischemia region where TTC was less uptake.The minipig with circumflex branch ligation was the better model for the myocardial infarction and circumflcx branch constriction for ischemia. ^99mTc-MIBI myocardial perfusion can define the infarction or ischemia segment in the living body while the TTC can also define in the isolated heart.