小鼠粗线期精母细胞钙离子通道膜片箝方法的建立

›› 2002, Vol. 22 ›› Issue (3): 138-140.

小鼠粗线期精母细胞钙离子通道膜片箝方法的建立

南京医科大学毒理学研究所神经毒理室,南京 210029

收稿日期:2002-04-30 出版日期:2002-01-25 发布日期:2013-03-19
基金资助:
国家自然科学基金资助项目（30170811）

Study on Ca²⁺+ Channels of Mouse Pachytene Spermatocytes Using Patch Clamp

Nanjing Medical University，Nanjing 210029,China

Received:2002-04-30 Online:2002-01-25 Published:2013-03-19

摘要/Abstract

摘要： 应用机械分离获得小鼠单个精母细胞，采用全细胞膜片箝技术记录Ca²⁺+离子通道电流。结果表明：①阻断K⁺电流后，当钳制电位-90mV、指令电压-60～+10mV、步阶电压10mV时，可记录到内向电流；②内向电流在-30～-40mV达到最大值；③在细胞外液中加入4μmol/LTTX，对记录电流无影响，表明此电流不含有Na⁺电流成分，为Ca²⁺+电流。

关键词: 精母细胞, 全细胞膜片箝技术, 钙离子通道

Abstract: Ca²⁺currents were obtained in acutely dissociated mouse spermatogenic cells using whole-cell patch clamp technique. The results indicated that inward currents were recorded when K⁺ currents were blocked by Cs⁺and TEA⁺stepping membrane potential to voltage between —80 and +10 mV，in 10 mV increments from a holding potential of —90 mV，the peak amplitude occurred at between —30 mV and —40 mV，and the inward currents remained unchanged in the presence of 4 μmol/L tetrodoxin （TTX）,a special sodium channels blocker，suggesting that it was generated by opening Ca²⁺channels other than Na⁺channels.

Key words: Spermatocyte, Whole-cell patch clamp technique, Ca²⁺channels

张莉，张习春，高蓉，肖杭. 小鼠粗线期精母细胞钙离子通道膜片箝方法的建立[J]. , 2002, 22(3): 138-140.

ZHANG Li, ZHANG Xi-chun, GAO Rong, XIAO Hang*. Study on Ca²⁺+ Channels of Mouse Pachytene Spermatocytes Using Patch Clamp[J]. , 2002, 22(3): 138-140.