Abstract: Employing the technology of antisense oligo-nucleotide acid,the authors established a mouse behavior monitoring platform aimed at the large scale screening of novel gene functions in CNS. And with this platform, 38 genes have been screened using BALB/c mice. Among them, 10 genes showed differences to the control groups （saline，scramble）. The authors screened the 10 genes again with C57BL/6 and ICR mice, and found the appearances were not always the same in three strains. In the first round screening, there are 6 of the 10 genes related to memory, 2 related to pain sensitivity,and the other 2 related to locomotion activity. But after the rescreening, only 3 genes show relativity with memory （Am , L, and Sn），and 2 genes show relativity with pain sensitivity （Pe, Rp）. The results indicate that the importance of using different strains in the experiments, and it is better to use inbred strains than outbred colonies in the screening of gene functions. The results suggested that the research on different appearances of different mouse strains in one experiment and the use of different mouse strains to testify one result are both having very important significance to animal experiments and laboratory animal science.