Abstract: To establish an allergic rhinitis model induced by ragweed pollen allergen, guinea pigs（n=42） were divided into a sensitized group（n=32）and an unsensitized control group（n=10）. Sensitized groups were immunized intraperitoneally and intracuta-neously with ragweed pollen allergen for 4 weeks, followed by intranasal administration. Unsensitized control group immunized with 0.9% NaCl instead of ragweed pollen allergen. As a results，symptoms of allergic rhinitis,such as sneezing，itching and rhinor-rhea were observed in 28 guinea pigs of sensitized groups. There were significant increases in the number of eosinophils and Mast cell in the epithelium after 30 min in the sensitized group received intranasal ragweed pollen allergen. So, An animal model of allergic rhinitis induced by ragweed pollen allergen was established successfully. It provides a tool for studying and treating allergic rhinitis’especially induced by ragweed pollen allergen.