实验动物与比较医学 ›› 2000, Vol. 20 ›› Issue (3): 138-140.

• 论文 •    

大鼠中脑切片的酪氨酸羟化酶和谷氨酸双重免疫荧光染色

  

  1. 福建医科大学分子医学教研室,福州 350004

Double Fluorescence Immunostaining for Tyrosine Hydroxylase and Glutamate in Rat Midbrain Sections

  1. Division of Molecular Medicine,Fujian Medical University,Fuzhou 350004,China

摘要: 在大鼠中脑组织切片上进行酪氨酸羟化酶和谷氨酸双重免疫荧光染色。用改良的脑灌注法以降低双重免疫荧光染色时谷氨酸的背景噪音,获得了满意的结果。在大鼠中脑腹侧,91%±4%的多巴胺神经元呈谷氨酸染色阳性,从细胞组织学上证明了中脑腹侧多数多巴胺神经元存在多巴胺与谷氨酸递质共存现象。

Abstract: Double fluorescence immunostain of tyrosine hydroxylase (TH) and glutamate (Glu) was used in midbrain sections of rat.With modified procedure of brain perfusion for reducing background of Glu staining,we got satisfied results,and found that 91%±4% of DA neurons were Glu positive.Cytohistological studies demonstrated that most ventral midbrain DA neurons were glutamatergic.