Abstract: To investigate the role of mannose and mannose receptor in sperm-zona binding in mouse. Capacitated spermatozoa, preincubated in the presence of D-mannose （DM） or D-mannosylated albumin （DMA），were co-incubated with zona-onclosed eggs pretreated with α-Mannosidase or Concanavalin A （ConA） respectively at the same time. The role of DM、DMA、α-Mannosidase、ConA in the interaction between mouse sperm and zona were studied by testing their effects on binding sperm to eggs. The results showed that the DM、DMA、α-Mannosidase、ConA decrease the number of sperm bound per zona pellucida. This findings suggested that mannose receptor may have a role in the interaction between sperm-zona.