›› 2000, Vol. 20 ›› Issue (3): 133-137.

• 论文 • 上一篇    下一篇

甘露糖及其相关分子对小鼠精子与透明带结合的影响

  

  1. 福建医科大学组织胚胎教研室,福州 350004
  • 收稿日期:2000-04-15 出版日期:2000-01-25 发布日期:2013-03-19

The Influence of Mannose and Mannose-related Regents on the Binding of Mouse Sperm-zona Pellucida

  1. Fujiang Medical University,Fuzhou 350004,China
  • Received:2000-04-15 Online:2000-01-25 Published:2013-03-19

摘要: 为探讨甘露糖及其受体在小鼠精子-透明带结合中的作用,小鼠附睾尾精子经M16工作液获能培养2h后,分别用DMA(甘露糖基化的牛血清白蛋白)、D-甘露糖处理15min,然后与未作处理的带透明带卵子共培养30min;另2组卵子分别用甘露糖苷酶、ConA(植物凝集素)处理15min后分别与未作处理的获能精子共培养30min。卵子固定,在相差显微镜下计数各组卵子的精子结合数。结果表明:DMA、D-甘露糖、甘露糖苷酶、ConA对精子和透明带结合有抑制作用。提示甘露糖及其相应受体在精卵相互作用中可能具有调节作用。

关键词: 小鼠, 精子, 透明带, 甘露糖受体

Abstract: To investigate the role of mannose and mannose receptor in sperm-zona binding in mouse. Capacitated spermatozoa, preincubated in the presence of D-mannose (DM) or D-mannosylated albumin (DMA),were co-incubated with zona-onclosed eggs pretreated with α-Mannosidase or Concanavalin A (ConA) respectively at the same time. The role of DM、DMA、α-Mannosidase、ConA in the interaction between mouse sperm and zona were studied by testing their effects on binding sperm to eggs. The results showed that the DM、DMA、α-Mannosidase、ConA decrease the number of sperm bound per zona pellucida. This findings suggested that mannose receptor may have a role in the interaction between sperm-zona.

Key words: Mouse, Spermatozoa, Zona pellucida, Mannose receptor