兔自体移植静脉基因转移模型的建立

实验动物与比较医学 ›› 2000, Vol. 20 ›› Issue (1): 3-6.

兔自体移植静脉基因转移模型的建立

上海第二医科大学 1.仁济医院心胸外科，上海 200001；2.人类基因治疗研究中心

收稿日期:1999-06-16 出版日期:2000-01-25 发布日期:2013-03-19
基金资助:
国家自然科学基金（编号 39570804）和卫生部科学研究基金（编号 96-1-335）

Establishment of Gene Transfer Model of Vein Autograft in Rabbit

1.Department of Cardiothoracic Surgery,Renji Hospital ,2.Human Gene Therapy Centre，Shanghai Second Medical University,Shanghai 200025

Received:1999-06-16 Published:2000-01-25 Online:2013-03-19

摘要/Abstract

摘要： 兔颈动脉-颈静脉旁路手术后，将阳离子脂质体DOSPER介导的p53腺相关病毒增强型质粒表达载体，经腔内加压灌注转染移植静脉。RTPCR和免疫组化检测证明，外源性p53基因在移植血管中表达出相应的mRNA及p53蛋白。结果提示：腔内加压灌注是移植静脉基因转移的有效方法之一。

关键词: 动脉旁路手术, 移植静脉, 基因转移

Abstract: The purpose of this study is to develop a new way to transfer extrinsic gene to vein graft. The models of carotid-jugular bypass were prepared in rabbits. The human wild-type p53 gene adeno-associated virus plasmid vector was transfected into vein graft mediated by the cationic liposome DOSPER by using intraluminal pressure perfusion method. The gene expression was determined by polymerase chain reaction technique and immunohistochemistry. It was verified that the extrinsic p53 gene was transduced into vein graft successfully and expressed mRNA and p53 protein. The results suggested that intraluminal pressure perfusion method is one of the efficient means for vein autograft gene transfer system.

Key words: Artery bypass, Vein graft, Gene transfer, Rabbit

梁宏立1,朱洪生1,黄忠耀1,等. 兔自体移植静脉基因转移模型的建立[J]. 实验动物与比较医学, 2000, 20(1): 3-6.

LIANG Hong-Li-1,ZHU Hong-Sheng-1,HUANG Zhong-Yao-1,et al. Establishment of Gene Transfer Model of Vein Autograft in Rabbit[J]. Laboratory Animal and Comparative Medicine, 2000, 20(1): 3-6.

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