›› 1999, Vol. 19 ›› Issue (3): 140-142.

• 论文 • 上一篇    下一篇

热休克对大鼠体内肝细胞ACP和AKP活性的影响

  

  1. 河南师范大学生物系,新乡 453002
  • 收稿日期:1998-08-11 出版日期:1999-01-25 发布日期:2013-03-19
  • 基金资助:

    中法技术合作项目

Effects of Heat Shock on Acid-and Alkaline-Phosphatase Activities in Rat Liver Cells

  • Received:1998-08-11 Online:1999-01-25 Published:2013-03-19

摘要: 观察了热休克反应大鼠其肝细胞酸性磷酸酶(ACP)和碱性磷酸酶(AKP)的活性变化。组织化学方法显示ACP和AKP活性的最适酶—底物作用时间分别为40min和2.5h;以不同温度(42、44和46℃)处理大鼠30min,室温恢复(25℃)24h时,42℃处理组的肝细胞ACP和AKP活性最强;46℃热休克30min后,室温恢复1~120h,在恢复至48h时,ACP和AKP活性最强。

关键词: 大鼠, 肝细胞, 热休克, 酸性磷酸酶, 碱性磷酸酶

Abstract: The activities of acid - and alkaline - phosphatase (ACP and AKP) in rat liver cells under normal and heat shock conditions were examined. The results showed that the best incubation time that enzymes interacting substrates is 40 min and 2.5h respectively. The rats were treated by heat shock at 42、44 and 46℃ respectively for 30min,then maintained at 25℃ for 24h,it was found that the activities of ACP and AKP in rat liver cells were the most active at 42℃ for 30 min. By heat shock of 46℃ for 30min ,and then at 25℃ for 1 -120h,ACP and AKP were the most active at 48h. The significance of ACP and AKP activi-tives responed to heat shock by rats was discussed.

Key words: "Hepatocytes, Heat shock, Acid phosphatase, Alkaline, phosphatase, Rat "