›› 1999, Vol. 19 ›› Issue (3): 133-136.

• 论文 •    下一篇

猕猴小肠5-羟色胺内分泌细胞的分布及形态学研究

  

  1. 河南师范大学生物系,新乡 453002
  • 收稿日期:1998-10-17 出版日期:1999-01-25 发布日期:2013-03-19
  • 基金资助:

    河南省动物学省级重点学科资助项目

Distribution and Morphology of the 5-HT Endocrine Cells in the Small Intestine of Rhesus Monkeys

  • Received:1998-10-17 Online:1999-01-25 Published:2013-03-19

摘要: 以免疫组织化学链霉卵白素(SP)法显示猕猴小肠5 羟色胺(5-HT)内分泌细胞,并用Weibel体视学方法对其进行了定量分析。结果表明,此种细胞的密度在十二指肠最高,空肠密度中等,回肠密度最低;5-HT免疫反应阳性细胞多分布在肠腺处,绒毛中较少。该细胞形态多样,大多为锥形、梭形、椭圆形或圆形。有的细胞基底部有突起,后者可伸向腺腔或肠腔,其末端含有5-HT阳性物质,提示此种细胞有旁分泌和外分泌功能。

关键词: 猕猴, 小肠, 5-HT内分泌细胞, 免疫组织化学方法, 体视学方法

Abstract: Six rhesus monkeys were used to study the distribution and morphology of 5 - hy-droxytryptamine (5-HT) endocrine cells in the small intestine by using immunohis-tochemical streptavidin/peroxidase (SP) method,and Weibel,s stereological method. The results were as follows:① the density of 5-HT endocrine cells in the small intestine of the rhesus monkeys was the highest in the duodenum and moderate in the jejunum and the lowest in the ileum;② 5-HT endocrine cells were distributed mainly in the glandulate duodenales and less in the villi;③ 5-HT cells were various in shape,mostly oval,shuttle, pyramidal or round ;and ④ basal portion of some endocrine cells had processes,extended to luminal surface of the crypt or intestinal tract with 5-HT positive substance accumulating in their ends. These results suggested that 5-HT endocrine cells have paracrine and exocrine functions.

Key words: "Rhesus monkey, Small intestine, 5-HT endocrine cells, Immunohis-tochemistry, Stereology "