实验动物与比较医学 ›› 1999, Vol. 19 ›› Issue (1): 29-30.

• 论文 •    

四种方法检测伯氏疏螺旋体IgG抗体比较

  

  1. 首都医科大学微生物学教研室,北京 100054

Comparative Study on Four Methods for Detecting Serum Antibody against Borrelia Burgdorferi

摘要: 用Dot-ELISA法、ELISA法、IFA法及CF法进行了检测兔莱姆病血清IgG抗体的比较实验。对10例人工感染伯氏疏螺旋体(莱姆病病原体)的兔血清的检测结果表明,Dot-ELISA法与ELISA法,IFA法及CF法的符合率分别为100%、90%和70%;对12例正常兔血清的检测,符合率均为100%。这提示,Dot-ELISA法可能成为一种用于检测人或动物莱姆病血清抗体的简便方法。

Abstract: Serum IgG antibody from rabbits artificially infected with Borrelia burgdorferi was detected with new — established Dot — ELISA,routine ELISA,IFA and CF, respectively. Ten serum samples from infected rabbits were examined,the results of Dot — ELISA showed 100%,90% and 70% coincidence with routine ELISA,IFA and CF respectively. while to 12 serum samples from healthy rabbits,it showed 100% coincidnce with ELISA, IFA and CF. All these suggested that Dot —ELISA may be a simple and convenient method for detecting serum IgG antibody against human and animal Lyme disease.