Abstract: To study the genetic characteristics of Zmu-1：DHP guinea pig,14 biochemical markers were analysed by electrophoretic method,and compared with those of the parent DHP strain. The results reveals that no polymorphism is showed on 10 loci in Zmu-1：DHP strain and 9 loci in DHP strain. Both strains manifest polymorphism on G-3-PD，G-6-PD,Es-6 and Es-8 loci，and GOT-1 locus in DHP strain is also polymorphic. There are a and b alleles on above 5 polymorphic loci and beside there is c allele on Es-8 locus in DHP strain.It also shows that the genotype on G-6-PD locus is sex related，most male guinea pigs possess b allele on G-6-PD locus while most females possess a allele on the same locus.