实验动物与比较医学 ›› 1993, Vol. 13 ›› Issue (3): 132-134.
• 论文 •
摘要： 对检测大鼠细小病毒（RV株）抗体的ELISA、IEA和HI三种方法作了比较。ELISA、IEA和HI法的重复性分别为92.5%、88.2%和68.6%。经对同一批血清检品的检测，ELISA法IEA和检测结果的符合率为90.5%，抗体阳性检出率均为81%，而HI与前两法的符合率均仅为69%，抗体阳性检出率为69%。以上结果表明：ELISA和IEA法在重复性和抗体阳性检出率方面均优于HI（P0.05）。经ELISA法检测，普通级大鼠中RV抗体阳性率达59%，而在清洁级大鼠中仅为7%。
Abstract: Comparison among ELISA, IEA and HI was made in detecting the antibody to rat Parvovirus.The reproducibilities of ELISA, IEA and HI were 92.5%,88.2% and 68.6% respectively. When the same sera were investigated,the consistencies of the results between ELISA and IEA, HI and ELISA/IEA were 90.5% and 69% respectively,and antibody-positive rates were 81% by both ELISA and IEA, but only 69% by HI. The reproducibilities of the seromonitoring results and the positive rates of antibody by ELISA and IEA were higher than by HI （P＜0.05）, RV antibody was found positive by ELISA in 59% of the conventional rat samples while in clean rat, the positive rate was only 7%.
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https://www.slarc.org.cn/dwyx/CN/Y1993/V13/I3/132
