检测大鼠细小病毒抗体的ELISA、IEA和HI法比较

贺争鸣,卫礼,张然,刘佐民,吴惠英

实验动物与比较医学 ›› 1993, Vol. 13 ›› Issue (3) : 132-134.

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实验动物与比较医学 ›› 1993, Vol. 13 ›› Issue (3) : 132-134.
论文

检测大鼠细小病毒抗体的ELISA、IEA和HI法比较

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Comparison among ELISA,IEA and HI for Detection of Rat Parvovirus Antibody

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摘要

对检测大鼠细小病毒(RV株)抗体的ELISA、IEA和HI三种方法作了比较。ELISA、IEA和HI法的重复性分别为92.5%、88.2%和68.6%。经对同一批血清检品的检测,ELISA法IEA和检测结果的符合率为90.5%,抗体阳性检出率均为81%,而HI与前两法的符合率均仅为69%,抗体阳性检出率为69%。以上结果表明:ELISA和IEA法在重复性和抗体阳性检出率方面均优于HI(P0.05)。经ELISA法检测,普通级大鼠中RV抗体阳性率达59%,而在清洁级大鼠中仅为7%。

Abstract

Comparison among ELISA, IEA and HI was made in detecting the antibody to rat Parvovirus.The reproducibilities of ELISA, IEA and HI were 92.5%,88.2% and 68.6% respectively. When the same sera were investigated,the consistencies of the results between ELISA and IEA, HI and ELISA/IEA were 90.5% and 69% respectively,and antibody-positive rates were 81% by both ELISA and IEA, but only 69% by HI. The reproducibilities of the seromonitoring results and the positive rates of antibody by ELISA and IEA were higher than by HI (P<0.05), RV antibody was found positive by ELISA in 59% of the conventional rat samples while in clean rat, the positive rate was only 7%.

关键词

酶联免疫吸附试验 / 免疫酶试验 / 血凝抑制试验 / 大鼠 / 细小病毒

Key words

ELISA / IEA / HI / Rat / Parvovirus

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贺争鸣,卫礼,张然,刘佐民,吴惠英. 检测大鼠细小病毒抗体的ELISA、IEA和HI法比较[J]. 实验动物与比较医学. 1993, 13(3): 132-134
Comparison among ELISA,IEA and HI for Detection of Rat Parvovirus Antibody[J]. Laboratory Animal and Comparative Medicine. 1993, 13(3): 132-134

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