Abstract: 12 loci （Cat, Es-l, Es-2, Es-3, Es-4, Es-5, Es-12, Es-C, Gl-l, Hbb, Svp-l, Trf） of Wistar inbred rats and SD outbred rats have been mornitored by authors using method of electrophoresis, and zymograms of 12 loci and conditions of electrophoresis have been established preliminarily. In this experiment, authors have observed and disscussed： （1） Es-1b can still be revealed when Es-2 occurs, but lighter than normal.（2） In rats, both of male and female can be found Es-3, and in a few male's, Es-3 be shown in testis, but not in small intestine,（3） For revealing Es-12, using small intestine is better than using heart.