Table of Content

31 March 2008, Volume 28 Issue 3

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The Animals and Emerging Infectious Diseases

QIN Chuan

2008, 28(3):  133-137. 

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The animals，from the surveillance reports on emerging infectious diseases, were main reservoir of pathogens. Many factors lead to the prevalent of zoonosis or emerging zoonosis emergence. The environmental changes, human and animal demography, pathogen changes and changes in farming practice are the most important factors triggering those diseases. The human social behaviors like food habits and religious beliefs play a role too. The harmonious between the humankind and animals and maintain the ecological balance are the important things we should do.

Study on the Feasibility of Establishing of rsp3111 Trangenic Mouse through in vivo Transfection of Spermatogonial Stem Cells

ZHOU Mi1,JIA Xiao-feng2,SHI Ting-yan2,WANG Jian2,YUAN Yao2,SHI Hui-juan2

2008, 28(3):  138-143. 

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Objective To construct a plasmid for fusion expression of rsp3111 protein and fluorescent protein, and to express rSP3111 protein in IVF embryos and in mature sperms in epididymis. Methods Expression plasmid pDsRed-Monomer-Nl -rSP3 111 was constructed by subcloning the cDNA of rSP3111 protein pDsRed-Monomer-Nl. Mouse sperms were transfected by expression plasmid pDsRed-Monomer-Nl-rSP3111 by liposome-mediated methods, and fertilized with mature eggs. Alternatively, pDsRed-Monomer-Nl -rSP3 111 plasmid were introduced into testis by liposome-mediated injection. The expression level and pattern of rSP3111 protein was examined by PCR assay and fluorescent microscopy. Results The result of PCR assays confirmed that rsp3111 gene was successfully tranfered into the sperms in epididymis and into fertilized eggs. The results of fluorescent microscopy shows the specific expression of rsp3111gene both in IVF embryos and in convoluted seminiferous tubules of the testis. Conclusion Specific expression of rat gamete-specific protein rSP3111 was achieved through sperm-mediated trasnfection, which undermines the establisment of rsp3 111 trangenic mouse through in vivo transfection of spermatogonial stem cells.

Production of α-1,3-GT Transgenic Mice Through Microinjection

LI Li,LIU Xi-mei,GU Xi-wen,XIAO Hong-wei,WANG Ya-gang,TIAN Yong-xiang,ZHENG Xin-min

2008, 28(3):  144-147. 

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Objective To product α-l，3-GT transgenic mice animal model for the study of xenograft. Method The α-l，3-GT genes was introduced into fertilized mice eggs of one-cell stage by microinjection. Result Eight hundred and fifty-eight fertilized eggs were introduced with α-l，3-GT gene. Of them, 312 fertilized mice eggs of one-cell stage were transplanted into 11 pseudpregnant mice, 6 of them were pregnanted (54.5%) and were aborted; 541 fertilized mice eggs of two-cell stage were transplanted into 23 pseudpregnant mice，18 of them were pregnanted (78.3%) and 11 pregnant females were aborted, 14 dead offspring were bom from three pregnant females. None were proved to be gene integration positively; 21 dead offspring were bom from four pregnant females of days or so, 7 of them were proved to be gene integration positively. Conclusion Transgenic mice have been obtained through microinjection of α-1, 3-GT genes, and further study needed to product the live ot-l，3-GT transgenic mice offspring.

Establish a High Rebeating Rate Mouse-to-rat Heterotopic Cardiac Transplantation Model

YE Wen-xue，SHEN Zhen-ya，ZHANG Sheng-chao

2008, 28(3):  148-150. 

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Objective To improve the operation skills of mouse-to-rat heterotopic cardiac xenotransplantation as well as to establish a high rebeating rate mouse-to-rat heterotopic cardiac transplantation model. Methods By the routine and improved operation procedures, One hundred and fifty heterotopic cardiac transplantation models were established. Results The rebeating rate in routine group and improved group were 81.43% (57/70)and92.50%(74/80) respectively. The total operation time of routine group was (72.57 士 10.52)min and donor operation time (7.73 土 1.90)min，and that of the improved group was (75.33 + 11.14)min(P＞ 0.05)and(8.96 + 1.93)min (P＜/i>＜0.05) respectively. Conclusion The improved procedure has advantages on improving rebeating rate of the model, though it needed more time of donor operation.

Study on Experimental Autoimmune Prostatitis in Rats

LI Dong-mei1,2,HU Wen-juan1,2,LIU Xiang-yun1,2,ZHANG Xiao-fang2,YAN Han2,XIE Shu-wu2,WU Jian-hui2,SUN Zu-yue2

2008, 28(3):  151-154. 

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Objective To establish an animal model of autoimmune prostatitis in Sprague-Dawley(SD) rats. Methods At the 0 and 7th day, male rats were immunized with a saline extract of male SD rat prostate glands(RPG) with the dosage of 20, 40 and 80g/L(intracutaneous) and Peitussis-Diphtheria-Tetanus vaccine 0.5 ml (peritoneal injection). At 21st day, male rats were immunized with 0.5 ml saline extract of rat prostate glands. At 35th day, the rats were sacrificed, and the prostate specimens，calcium ion, testosterone(T) and dihydrotestosterone(DHT) were observed or determined. Results Compared with control group, WBC counts increased obviously in high-dosage model group, its interstitial tissue and glandular cavities were full of akaryocytes, lymphocytes and monocytes. The high-dosage model group appeared chronic inflammation response, calcium ion obviously decreased. Conclusion Thrice and multipoint injecting 80 mg/ml RPG with FCA and twice injecting 0.5 ml Pertussis-Diphtheria-Tetanus vaccine was an effective method for establishing autoimmune prostatitis model in SD rats.

The Study of Tibet Mini-pigs Myostatin Gene in 5' Controllable Region by PCR-RFLP

YU Min1,GU Dong-sheng2,LI Hong-tao1,ZHANG Jian-ming1,LI Jin-ze1,WANG Yu-jue1,GU Wei-wang1

2008, 28(3):  155-159. 

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Objectives To analyze the MSTN gene of Tibet mini-pigs，WU Zhi-shan mini-pigs，Bama mini-pigs and Guizhou mini-pigs.Methods Taking 42 Tibet mini-pigs，17 Wu Zhi-shan mini-pigs，7 Bama mini-pigs and 22 Guizhou mini-pigs as samples，PCR-RFLP polymorphism analysis on the controllable region of the MSTN gene 5’of pigs were carried out.42 Tibet mini-pigs were used to analyze the relation between the RFLP polymorphism of MSTN gene and some breeding characters（weight at birth（WB）and weaning weight（WW））.Results（l）There is significant difference in genetype frequencies and allele frequencies between different stocks of pigs（P=0.000）.Tibet mini-pigs have three MSTN genetypes TT（9.52%）、AA（61.90%）、TA（28.57%）、and the frequencies of alleles T and A is 23.81%and 76.19%respectively；Wu Zhi-shan mini-pigs have two genetypes TT（82.35%）、AA（17.65%）、and the frequencies of alleles T and A is 82.35%and 17.65%respectively；Guizhou minipigs have two genetypes TT（81.82%）、TA（1 8.18%）、and the frequencies of alleles T and A is 90.91%and 9.09%respectively；Bama mini-pigs have two genetypes TT（71.43%）、TA（28.57%）、and the frequencies of alleles T and A is 85.71%and 14.29%respectively.（2）The difference of WB（P=0.761）and WW（P

Study on PERV Gene in Four Stocks of Mini-pigs

LI Jin-ze,YUE Min,ZHANG Jian-Ming,Wang Yu-Jue,GU Wei-Wang

2008, 28(3):  160-163. 

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Objective Estimate difference of PERV copy-number of mini-pigs in China, and to find out breeds or individuals with no or low vims copy-number. Methods Semi-quantitative PCR method was used in this research to estimate PERV copy-number of four mini-pigs in China. Results The copy-number of PERV, EnvA, EnvB gnes in Guizhou mini-pig, Wuzhishan mini-pig, Tibet mini-pig, Bama mim-pig were 34.12±16.96, 29.38±13.83, 28.71±14.11，27.12±14.43(F=0.614, P = 0.609)， 9.78 士 5.48，10.06 土 5.34，10.23 土 5.71，10.51 土 6.23(F=0.044，P = 0.988)，24.14 土 11.26， 20.72 士 8.36，18.35 土 8.50，17.60 土 8.65(F=1.512，P＜/i>=0.221) respectively. According to these results, no significant differences were observed in the copy-number of PERV, EnvA, EnvB among four different mini-pig breeds. Conclusion The four stocks of commonly used mini-pigs have relatively low endogenous vims copy-number, and it may be used for xenotransplantation research.

Dynamic Changes of Blood Biochemical Indexes of Tibet Minit-pigs During Estrous Cycle

Nashunbayaer,YU Juan,CHEN Li,WU Qing-hong,GU Wei-wang

2008, 28(3):  164-166. 

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Objective To study the dynamic changes of blood biochemical indexes of Tibet mini-pigs during the estrous cycle. Methods Take blood sample from precava of 6 female Tibet mini-pigs for testing the blood biochemical indexes, including all the indexes changed during estrous cycle, and what’s more the difference of AST between 9th and 20th day, and the difference of CHO between 19th day and 10th, 12th, 13th day was significant(P＜0.05). Besides, at different stage of estrous cycle, dynamic change of the level of Ca, Mg, ALP, Cre, CHO, TP, and Alb were obviously. Conclusion The biochemical indexes of Tibet mini-pigs have obviously dynamic change at different stage of estrous cycle, it could be useful for related biomedical researches.

Establishment of Melioidosis Model with FMMU Albino Guinea Pig

WANG Wan-shan1,LIU Zhen-xi2,ZHANG Jia-ning1,WU Qing-hong1,GU Wei-wang1

2008, 28(3):  167-170. 

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Objective To establish melioidosis model of FMMU albino guinea pig. Method FMMU albino guinea pig infection model were made by intraperitoneal inoculation of Burkholderia pseudomallei. The clinical manifestations, the amount of bacteria in blood，mortality rate and histopathologic changes in major parenchymatous organs were observed, the killing effect of polymorphonuclear leukocyte on Burkholderia pseudomallei was observed by electron micro scope (EM). Result The characteristic sign of the melioidosis appeared after inoculation, the amount of bacteria in blood was 1 4000 CFU/ml at 72 h post inoculation，mortality rate was 70% at 96 h post inoculation. Under light microscope there were typical suppuration focus in parenchymatous organ. Polymorphonuclear leukocyte could kill the Burkholderia pseudomallei when observed under EM. Conclusion Burkholderia pseudomallei infection model of FMMU albino guinea pig was established successfully and may be used for research on acute Melioidosis in human.

Effects of Alfalfa Extract on Experimental Hyperlipemia in Rats

YU Juan,Nashunbayaer,YANG Hai-ying,CHEN Li,GU Wei-wang

2008, 28(3):  171-173. 

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Objective To establish hyperlipemia animal model and investigate the functional changes of alfalfa extract in hyperlipemia rats. Methods 40 male SD rats were randomly divided into 4 groups after fed with high-fat and high-cholesterol diet for 3 weeks, The model group(?=10), fed with high-fat and high-cholesterol diet: the low, middle and high intervention group was added with alfalfa extract at the dosage of 0.60，1.20 and 3.60 g/kg respectively. Besides other 10 male rats as negative control group fed with normal diet.All the rats were sacrificed after 35 days. The TC、TG、HDL-C levels and the pathological changes of the liver, heart, kidney, lung and artery were detected. Results TC、TG levels were rose after fed with high-fat and high-cholesterol diet and decreased after added with alfalfa extract. Pathological changes lessened in intervention group. Conclusion The alfalfa extract can reduce the TC、TG、HDL-C levels and pathological changes of the liver in hyperlipemia model rat.

Advances on Spermatozoa Cryopreservation in Rat

GAO Juan1,2,JIA Qing1,2,YANG Bo2,3,LIU Li-jun2,YU Li-li2,RUI Rong1,XU Ping2

2008, 28(3):  195-198. 

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Spermatozoa cryopreservation is an efficient and economical method of the gene resource preservation. Now, many mammary animals spermatozoa cryopreservation protocols have been established and be used wildly. Whereas, as the special structure of rat spermatozoa, the successful cryopreservation protocol of its spermatozoa is very difficult. The article made a simple review on the advances of spermatozoa cryopreservation in rat.