Abstract: Objective To establish an animal model of autoimmune prostatitis in Sprague-Dawley(SD) rats. Methods At the 0 and 7th day, male rats were immunized with a saline extract of male SD rat prostate glands(RPG) with the dosage of 20, 40 and 80g/L(intracutaneous) and Peitussis-Diphtheria-Tetanus vaccine 0.5 ml (peritoneal injection). At 21st day, male rats were immunized with 0.5 ml saline extract of rat prostate glands. At 35th day, the rats were sacrificed, and the prostate specimens，calcium ion, testosterone(T) and dihydrotestosterone(DHT) were observed or determined. Results Compared with control group, WBC counts increased obviously in high-dosage model group, its interstitial tissue and glandular cavities were full of akaryocytes, lymphocytes and monocytes. The high-dosage model group appeared chronic inflammation response, calcium ion obviously decreased. Conclusion Thrice and multipoint injecting 80 mg/ml RPG with FCA and twice injecting 0.5 ml Pertussis-Diphtheria-Tetanus vaccine was an effective method for establishing autoimmune prostatitis model in SD rats.