Abstract: Objective To establish hyperlipemia animal model and investigate the functional changes of alfalfa extract in hyperlipemia rats. Methods 40 male SD rats were randomly divided into 4 groups after fed with high-fat and high-cholesterol diet for 3 weeks, The model group(?=10), fed with high-fat and high-cholesterol diet: the low, middle and high intervention group was added with alfalfa extract at the dosage of 0.60，1.20 and 3.60 g/kg respectively. Besides other 10 male rats as negative control group fed with normal diet.All the rats were sacrificed after 35 days. The TC、TG、HDL-C levels and the pathological changes of the liver, heart, kidney, lung and artery were detected. Results TC、TG levels were rose after fed with high-fat and high-cholesterol diet and decreased after added with alfalfa extract. Pathological changes lessened in intervention group. Conclusion The alfalfa extract can reduce the TC、TG、HDL-C levels and pathological changes of the liver in hyperlipemia model rat.

Key words: Hyperlipemia, Alfalfa extract