Abstract: Objective Estimate difference of PERV copy-number of mini-pigs in China, and to find out breeds or individuals with no or low vims copy-number. Methods Semi-quantitative PCR method was used in this research to estimate PERV copy-number of four mini-pigs in China. Results The copy-number of PERV, EnvA, EnvB gnes in Guizhou mini-pig, Wuzhishan mini-pig, Tibet mini-pig, Bama mim-pig were 34.12±16.96, 29.38±13.83, 28.71±14.11，27.12±14.43(F=0.614, P = 0.609)， 9.78 士 5.48，10.06 土 5.34，10.23 土 5.71，10.51 土 6.23(F=0.044，P = 0.988)，24.14 土 11.26， 20.72 士 8.36，18.35 土 8.50，17.60 土 8.65(F=1.512，P＜/i>=0.221) respectively. According to these results, no significant differences were observed in the copy-number of PERV, EnvA, EnvB among four different mini-pig breeds. Conclusion The four stocks of commonly used mini-pigs have relatively low endogenous vims copy-number, and it may be used for xenotransplantation research.