Abstract: Objective To improve the operation skills of mouse-to-rat heterotopic cardiac xenotransplantation as well as to establish a high rebeating rate mouse-to-rat heterotopic cardiac transplantation model. Methods By the routine and improved operation procedures, One hundred and fifty heterotopic cardiac transplantation models were established. Results The rebeating rate in routine group and improved group were 81.43% (57/70)and92.50%(74/80) respectively. The total operation time of routine group was (72.57 士 10.52)min and donor operation time (7.73 土 1.90)min，and that of the improved group was (75.33 + 11.14)min(P＞ 0.05)and(8.96 + 1.93)min (P＜/i>＜0.05) respectively. Conclusion The improved procedure has advantages on improving rebeating rate of the model, though it needed more time of donor operation.