Table of Content

25 January 2006, Volume 26 Issue 1

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Effects of Sodium Cholate on Pregnant Ewes and Fetuses

LI Xin-Ping-1, LI Jing-1, CHEN Long-2, MAO Xin-Zhi-3

2006, 26(1):  10-15. 

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Objective To investigate the effects of sodium cholate on the mother and fetuse in intrahe-patic cholestasis of pregnancy （ICP）. Method Pregnant ewes were served as animal models. High dose of sodium cholate was infused to pregnant ewes through jugular fistula. Results The pruritus, fetal distress, and intrauterine death appeared in the animal of treated group, which were similar with the symptoms of pregnancy women in ICP. Conclusion The sodium cholate could influence on the mother and fetuse of the ewes，and further confirmed sodium cholate was an important factor to induce symptoms of ICP.

Different Effects of Recombinant Nattokinase on Coagulation and Fibrinolysis System in Beagle Dog and Miniature Swine

LI Xin-Zhi, LIU Jian-Xun, SHANG Xiao-Hong

2006, 26(1):  16-18. 

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Objective To study the different effects of recombinant nattokinase （r-NK） on coagulation and fibrinolysis system in Beagle dogs and in miniature swine when trqated repeatedly at large dosage. Methods Platelet-related parameters were obtained by blood analyzer. The fibrinogen （Fib）, activated partial thrombin time （APTT），prothrombin time （PT） and thrombin time：（TT） were determined by coagulation method with the coagulation detector. Plasmmogen （PLG） and tissue-type plasminogen activator （t-PA） were tested by ELISA. Results Repeated administration presented the same blood vessel irritation both in Beagle dogs and miniature swine. After 1 week of administration, the number of platelets was decreased, PT was prolonged, Fib concentration decreased and the activity of PLG were increased in Beagle dogs. However, r-NK had no effects on the above parameters in miniature swine. Conclusion There was toxic difference between different animals towards r-NK at the same dosage. In Beagle dogs, r-NK could break the balance between the coagulation and fibrinolysis system. Whereas r-NK had no effects on the coagulation and fibrinolysis system in miniature swine.

Model Establishing of Rat Skin Wounds Covered with hVEGF Genetically Engineering Membrane

ZHAO Lei-1, WANG Li-1, JIANG Xu-Cheng-1, ZHENG Lin-1, ZHANG Fan-1, ZHAO Han-Fang-2, XU Wei-Rong-2

2006, 26(1):  19-22. 

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Objective To establish a model of rat skin wounds which was covered with the genetically engineering membrane planted by those integrated with hVEGF recombinant plasmid. Method Wounds with full-thickness cutaneous defect were produced on the dorsum of the SD rats. The rats were randomly divided into four groups. For the rats in the experimented group, the wounds were covered with hVEGF genetically engineering membrane. For the three control groups, the wounds were covered with membrane planted by the fibroblast integrated with blank plasmid, blank membrane and Tegaderm mem-brane respectively. The specimens were obtained at the 3,7,14 and 29 days after injury, and examined histopathologically. Results Compared to that of Control groups, the hyperplasia of fibroblast and new capillary vessel in rat skin wounds were increased at all different time after injury in experiment group.Conclusion Vasculogenesis and granulation tissue growth were accelerated in skin wound which was covered with the genetically engineering membrane planted by those integrated with hVEGF recombinant plasmid.

Survival of Two-step Open-pulled Straw Vitrification of Mouse Eight-cell Embryos

ZHOU Guang-Bin-1, 3 , HOU Yun-Peng-1, YANG Zhong-Qiang-1, YANG Qi-恩1, LI Xiu-Wei-1, ZHU Shi-恩1, 2

2006, 26(1):  23-27. 

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Objective To establish potentially an effective and easy method for the vitrification of embryos from laboratory animals, domestic animals and human . Methods The experiments were conducted at 37℃ hot plate and 25℃ room temperature to cryopreserve mouse eight-cell embryos with EDFS30 （an ethylene glycol-based vitrification solution） in Open-pulled Straw （OPS）. In the two-step OPS method, the superovulated embryos were first pretreated with 10% EG+10% DMSO for 30s, then exposed to EDFS30 for 15s, 25s, 35s, 45s or 60s and then immersed in liquid nitrogen. After warming, the survival of embryos was assessed by their development to blastocysts or to term after transfer. Results The higher blastocyst rates of vitrified embryos were 95.6%, which were similar （P＞0.05） to that （93.0%） of control. The embryos derived from the best vitrified group or fresh eight-cell embryos （used as control） after culture for 1 to 3h were transferred to 63 to 67h pseudo-pregnant female mice. Twenty-three fetuses were obtained from 4 of 11 recipients which had received 165 vitrified-warmed embryos. In the pregnant recipients, the percentage of transferred embryos developed to young in the treated group （38.3%） and control （37.9%） showed no significant difference （P＞0.05）. Conclusion The mouse eight-cell embryos could survive cryopreservation by vitrification with EDFS30 in OPS.

Effect of Nutrient Restriction on Triglyceride Content and Bone Mineral Density in Aged Mice

SHEN Zhi-Xiang-1, QIAO Wei-Wei-2, LIU Cui-Xian-1

2006, 26(1):  28-30. 

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Objective Effects on the lipid and bone metabolism were discussed by nutrient restriction in aged mice. Methods The male KM mice of 12 month age were used for the nutrient restriction plan that 30% off total energy a day during 12 weeks. The same amounts of 3,7 and 12 month age, male KM mice were used as the young, adult and aged control group respectively. The changes of body weight, triglyceride （TG） content and bone mineral density （BMD） were tested. Resu. ts There was a significant decrease of body weight in the restriction group in the second, third week, then become a slow decrease. Compared with the aged control, TG was lower in the restriction group （P＜0.05）. Compared with the adult and aged control group respectively, there was no significant difference on BMD of the restriction group. Conclusions 30% off nutrient restriction plan induced the TG decreasing significantly and BMD was influenced by the synthetic factors in bone metabolism.

Observation on Myocardial Bridge in Dog and Comparison with Human

SHEN Biao-1, GUAN Yong-Qiang-2, GUO Zhi-Kun-1, XUE Bing-2, ZHANG Xiao-Yan-2

2006, 26(1):  31-33. 

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Objective To provide evidence for establishing animal model, and to investigate the effects of myocardium bridge on ischemic myocardium.Methods The left and right coronary artery and their branches of 41 cases of dogs’ hearts were anatominged and observed. Results Myocardium bridges appeared on dogs’ hearts in 29 cases out of 41 cases；1、2、3、4、5 myocardium bridges appeared respectively in 14 cases （of 34.1%）、7 cases （of 17.1%）、6 cases （of 14.6%）、1 cases （of 2.4%）、1 cases （of 2.4%）.The myocardium bridge appeared on anterior interventricular，posterior interventricular branch, on anterior branch of the left venteicle,on posterior branch of the left ventricle, on the left marginal branch,and the left branch of conus 1 leases （26.83%）, 5 case （12.20%）, 3 cases （7.32%）, 2 cases （4.88%）, 1 cases （of 2.44%） respectively.The angle between the fibers of the myocardium bridge and nural coronary artery is 68.94°±14.38°,the width of the myocardium bridge is 7.77土5.05 mm,and the thickness of the myocardium bridge is 0.56士0.61 mm.Conclusion Dogs’ myocardium bridges are similar to that of human,so dog is the ideal modles for studying the effects of myocardium bridge on ischemic myocardium.

Effect of Frozen Methods and Cryoprotections on Quality of Frozen Sperm from Beagle Dog

ZHANG Jiang-1, QIAO Wei-Wei-2, GU Jian-Xin-1, YU Jin-Guan-1, ZENG Hui-1, PAN Hua-2

2006, 26(1):  34-37. 

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Objective To assess the effect of frozen methods and cryoprotections on quality of frozen sperm from Beagle dog. Methods Five male Beagle dogs were chosen for collection of semen and 25 fractions of semen samples were diluted with three kinds of extenders and frozen separately using two methods. Result Average post thaw progressive motility were 51.9% and 53.9，and the percentage of sperms with intact acrosome were 36.4% and 37.6%, respectively. The percentage of sperms with intact acrosome supplemented with protect preservative agent Ⅲ were 40.7 and 41.3 by the frozen-straw method and pellet method, which better than the other agent, but only the percentage of sperms with intact acrosome was significant （P＜0.05）. Conclusion The frozen-straw method was better than the pellet method, but there was no statistical difference. The extender supplemented with vitamin E, B6 and glutamic acid is superior to the other two extenders in the average post thaw progressive motility, the percentages of recovery sperms and the percentage of sperms with intact acrosome, but only the percentage of sperms with intact acrosome was significant （P＜0.05）.

Advances and Evaluation on Research of Animal Models of Alzheimer's Disease

XIE Ning, SONG Lin-Li, NIU Ying-Cai, WANG Miao, JI Ren-Jun, DAI Xiao-Guang

2006, 26(1):  50-53. 

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AD is a kind of age-related degeneration disease in nervous system.The research of animal models of Alzheimer’s disease is restrictive because it’s cause isn’t clear and etiopathogenesis The authors reviewed and evaluated various kind of AD animal models and concluded that the Tg2576 mice had been a suitable animal model for testing of novel anti-AD drugs.

Hypertrophic Cardiomyopathy Animal Model and Application of Banna Minipig Inbred Line

YAO Li-Xin-1, CHEN Yi-Yue-1, ZENG Yang-Zhi-2

2006, 26(1):  54-58. 

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The pathogeneses and current situation of animal models of hypertrophic cardiomyopathy was reviewed. With the unique inbred genetic backgrounds and resemblance with what of human beings in anatomy and physiology, the prospective of the application of Banna Minipig Inbred Line as the model of hypertrophic cardiomyopathy was explored.