Table of Content

25 December 2004, Volume 24 Issue 4

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Experimental Study on Rabbit Atherosclerotic Plaque Rupture Model

CAI Bao-Xiang, XIE Mei-Lin, GU Zhen-Lun

2004, 24(4):  200-203. 

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To establish atherosclerotic animal model whose plaque could be evoked into rupture, male New Zealand white rabbits were fed with a high-cholesterol diet for 2 weeks, then underwent balloon-induced arterial wall injury, and fed with a high-cholesterol diet for 6 weeks and a regular diet for 8 weeks. Plaque rupture and thrombosis were triggered by injection of Russell's viper venom （0.12 mg/ kg,ip） and histamine （0.016 mg/kg,iv）. The area of thrombosis on atherosclerotic aorta, morphological character of plaque rupture, levels of blood lipid and lipoproteins were determined by image analysis, electron and light microscope, chromatometry, respectively. The results showed that the surface area covered by thrombus was 0.9±1.1 mm² in normal group and 77.7± 53.8 mm² in model group.Microscopy of arterial samples from model group showed obvious ulcers occurred and inflammatory cells infiltrated in shoulder area of plaque.Macrophages and lipid were rich in plaque. Content of cholesterol in abdominal aorta was 6.6 ±1.4 mg/g wet tissue in normal group and 24.2±7.7 mg/g wet tissue in model group. Conclusions：This animal model mimicked atherosclerotic plaque rupture and thrombosis，and could be used in study on mechanism of plaque rupture and screening drugs of plaque stabiligation out relational compound.

Establishment of Allergic Asthma Model in Guinea Pigs

GONG Zhao-Hua-1, DONG Jing-Cheng-1, NI Jian-2

2004, 24(4):  204-206. 

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To establish the allergic asthma model, guinea pigs were intraperitoneally injected with ovabulmin, and challenged by inhaling ovabulmin with ultrasonically nebulised inhaler two weeks later. The total number of cell and eosinphil count in brochial alveolar lavage fluid （BALF）, eosinphil count in plasma, airway resistance,pathology were detected. The results showed that eosinphil count in plasma and BALF of the model animal were significantly increased after the challenge compared with the control, intrapul-monary pressure were increased after inhaling histamine, and eosinphil imflammion were found in patho-logical exanination.

Establishment of Lead Poisoning Model in Neonatal Mongolian Gerbil

WEI Yong-Fang, DAI Li-Jun, HUANG Li, LIANG Cheng-Jie

2004, 24(4):  207-210. 

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To establish the neonatal Mongolian gerbil model of lead poisoning, eleven litters of 6-7days neonatal Mongolian gerbil were randomly divided into normal，low and high lead group. The animals were injected intraperitoneally with 0.9% saline or lead acetate trihydrate at the doses of 12,36 mg/kg （according to bodyweight） every other day and were breastfeeding by their mother.The weight of body and brain,lead density of blood and haemoglobin of neonatal Mongolian gerbil were measured. The pathological changes of kidney and brain were observed. The results showed thatthe weight of body,brain, and haemoglobin in low-lead and high-lead group were remarkably decreased （P＜0.05, P＜0.01） comparing to the control group,there are negative correlation between the bodyweight and lead density of blood （r=-0.669, P＜0.05）, brain weight and lead density of blood （r=-0.7403, P＜0.01）,and also haemoglobin and lead density of blood（r=-0.919；P＜0.01）； the lead density of blood was significantly increased （P＜0.01 ），and there were positive correlation between the lead density of blood and doses of lead （r=0.917, P＜0.01）.The kidney and brain damages were observed in light microscope, proximal tubule epithelial cell appeared swelling and degeneration, nervous cell appeared vacuolation necrosis and pyramidal cell degeneration in lead poisoning animals. It is suggested that the method for establishment of lead poisoning model of neonatal Mongolian gerbil by intraperitoneal injection was a kind of simple,stable and controllable way, and the model may be used for study of early stage nervous system and blood vessel system.

Establishment of Monitoring Methods of Biochemical Markers in Meriones Unguiculataus

SUN He-Juan-1, WANG Dong-Ping-1, WANG Ju-2, LI Gui-Jun-1, CHEN Zhen-Wen-2

2004, 24(4):  211-213. 

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To establish monitoring methods of biochemical markers in Meriones Unguiculataus，the biochemical markers of Meriones Unguiculataus were screened，and its electrophoretic conditions of isoenzymes and proteins were probed according to cellulose acetate membrane electrophoresis of proteins and isoenzymes. A total of 28 suitable biochemical markers were screened out as regular genetic monitoring for Meriones Unguiculataus, and appropriate tissue, run time and voltage of electrophoresis were determined. The Monitoring methods of biochemical markers established may be useful for genetic quality analysis of Meriones Unguiculataus.

An Elementary Study on Genetic Diversity of Z:ZCLA Mongolian Gerbil Basing on Microsatellite DNA Marker

LIU Yue-Huan-1, SA Xiao-Ying-1, WU Jiu-Sheng-2, SHI Zhang-Kui-1, YU Qiang-1

2004, 24(4):  214-217. 

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The polymorphism of 9 microsatellite loci was detected in Z：ZCLA Mongolian gerbil from Zhejiang center of laboratory animal , Zhejiang Academy of Medical Sciences. The results showed that 8 loci were polymorphic and locus 1 had 1 allele, and others had 2.4 alleles. The average of alleles is 2.6.

Detection of Rodent Coronavirus by Using Reverse Transcriptase Polymerase Chain Reaction

LIU Zhong-Hua, HUANG Ren, WEI She-Lin, LIU Xiang-Mei, CHEN Mei-Li

2004, 24(4):  218-220. 

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In order to detect rodent coronavirus, a pair of PCR primers were designed according to the compared sequences of rodent coronavirus and SARS coronavirus. The primers derived from the specific and conservative N gene，RT-PCR method was established. Result of rodent RT-PCR amplification showed the amplified products of 148bp length； RT-PCR method of rodent coronavirus has been used to detect the samples. The rodent RT-PCR method is important to determine early rodent coronavirus and discriminate SARS coronavirus.

Preliminary Study on Mandible Variables of KM,BALB/c,ICR Mice

CHEN Zhe, ZHAO Lin, CHEN Xiao, WU Su-Hui, ZHAO Xiao-Jin

2004, 24(4):  221-223. 

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In the present paper ,11 variables of mandibles of adult male mice from three strains were measured. The mice involved were 16 KM，10 BALB/c, 10 ICR respectively.The variables of mandibles were analyzed by Enter Independents Together and Use Stepwise Method of SPSS for Windows 10.0. These variables were used to establish discriminant functions for distinguishing among the three differ-ent strains. The correct discriminant rates both reach 100% by original grouped cases；the correct discriminant rates both reach 97.2% by cross-validated grouped cases.

Effect of Coronary Stent Implantation on Coagulation and Platelet Function of Rabbits

ZHAO Yi-Ming, JI Shun-Dong, ZHANG Wei, HE Yang, SHEN Wen-Hong, RUAN Chang-Geng

2004, 24(4):  224-227. 

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To study the effect of coronary stent implantation on the coagulation and platelet function of rabbits, eighteen New Zealand white male rabbits were underwent precutaneous transluminal coronary stenting. Their blood samples were collected before and at 1，2，4，24 hour after the stent implantation, respectively. The coagulant parameters of thrombin time （TT）, prothrombin time （PT）, activated partial thromboplastin time （APTT） and the fibrinogen, P-Selectin and thromboxane B₂ （TxB₂） level in plasma, platelet number, and tested platelet aggregation function were detected. The coagulation parameters of TT, PT, APTT had no significant change after the stent implantation, but the platelet number and the fibrinogen level in plasma was decrease after stent implantation and came back to the normal level in the 24 hour after the surgery. The level of platelet aggregation rate and P-Selectin and TxB₂ in plasma was higher after the stent implantation. Therefore, the coronary stent implantation had significant effect on the platelet activation，thrombosis and the activation of congulant system, It was approved that it is necessary to inspect the platelet activation and therapy with antithrombotic medicine after the stent implantation simultaneously.