›› 2004, Vol. 24 ›› Issue (4): 218-220.
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Abstract: In order to detect rodent coronavirus, a pair of PCR primers were designed according to the compared sequences of rodent coronavirus and SARS coronavirus. The primers derived from the specific and conservative N gene,RT-PCR method was established. Result of rodent RT-PCR amplification showed the amplified products of 148bp length; RT-PCR method of rodent coronavirus has been used to detect the samples. The rodent RT-PCR method is important to determine early rodent coronavirus and discriminate SARS coronavirus.
Key words: Reverse Transcriptase polymerase chain reaction, Rodent Coronavirus, SARS Coronavirus
LIU Zhong-Hua, HUANG Ren, WEI She-Lin, LIU Xiang-Mei, CHEN Mei-Li. Detection of Rodent Coronavirus by Using Reverse Transcriptase Polymerase Chain Reaction[J]. , 2004, 24(4): 218-220.
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https://www.slarc.org.cn/dwyx/EN/Y2004/V24/I4/218