Preliminary Studies on Biochemical Loci of Naked Mole Rat

doi:10.3969/j.issn.1674-5817.2013.06.014

Abstract

Abstract: Objective A elementary detection method on biochemical marker loci was established to study the characteristics of naked mole rat biochemical loci. Methods With inbred mouse as a reference, 11 isozymes of naked mole rat were detected according to the methods for biochemical markers of mice. Results The esterase-1, transferrin of naked mole rat showed slower bands; glucose phosphate isomerase-1, malic enzyme-1, isocitrate dehydrogenase-1 showed intermediate bands; hemoglobin chain, peptidase-3, alkaline phosphatase-1, phosphate glucose mutase-1, esterase-3, hydrogen peroxide enzyme-2 showed faster bands. Conclusion Biochemical locis of 7 naked mole rat performed consistent bands, showed homozygous, no polymorphism.

Key words: Naked mole rat, Biochemical loci of naked mole rat, Genetic monitoring

LIN Li-fang, WANG Hong, ZHAO Shan-min, XIAO Bang, SUN Zhi-yuan, ZHANG Lu, YUAN Zi-yan, YU Chen-lin, CAI Li-ping, XU Chen, TANG Qiu, SUN Wei, CUI Shu-fang. Preliminary Studies on Biochemical Loci of Naked Mole Rat[J]. Laboratory Animal and Comparative Medicine, 2013, 33(6): 477-480.

References

[1] Jarvis JUM.Ecological studies on Hererocephalus glaber, the naked mole-rat, in Kenya[J]. Natl Geogr Res Rep, 1985, 20:429-437.
[2] Faulkes CG, Abbott DH, Jarvis, JU.Social suppression of ovarian cyclicity in captive and wild colonies of naked mole-rats, Heterocephalus glaber[J]. J Reprod Fert, 1990, 88(2): 559-568.
[3] Buffenstein R.Negligible senescence in the longest living rodent,the naked mole-rat:insights from a successfully aging species[J]. J Comp Physiol B, 2008, 178(4):439-445.
[4] Buffenstein R,Jarvis JU.The Naked mole-rat-a new record for the oldest rodent[J]. Sci Aging Knowledge Environ, 2002,2002(21):pe7.
[5] GB/T 14927.1-2008实验动物近交系小鼠、大鼠生化标记检测方法[S].
[6] 孙伟, 汤球, 赵善民, 等. 裸鼹鼠人工饲养繁殖初步研究[J]. 实验动物与比较医学, 2013, 33(4):296-300.
[7] 林丽芳, 赵懿宁, 赵善民, 等. 裸鼹鼠与C57BL6小鼠自噬调节的比较研究[J]. 实验动物与比较医学, 2013, 33(4):301-305.
[8] 袁子彦, 赵懿宁, 张璐, 等. 裸鼹鼠肺脏显微结构与超微结构观察[J]. 实验动物与比较医学, 2013, 33(5):378-382.
[9] 袁子彦, 赵懿宁, 张璐, 等. 裸鼹鼠肝脏显微结构与超微结构观察[J]. 实验动物与比较医学, 2013, 33(5):373-377.
[10] 赵善民, 赵懿宁, 汤球, 等. 裸鼹鼠胸腺、脾脏及淋巴结解剖学、组织学与超微结构研究[J]. 实验动物与比较医学, 2013, 33(5):395-399.
[11] 赵善民, 崔淑芳. 新型实验动物裸鼹鼠生物学特性的研究进展[J]. 实验动物与比较医学, 2013, 33(5):400-405.
[12] 张璐, 赵懿宁, 袁子彦, 等. 裸鼹鼠与C57BL/6J小鼠肾脏结构及超微结构比较[J]. 实验动物与比较医学, 2013, 33(5):388-394.
[13] Bahr A, Wilson AB.The evolution of MHC diversity: evidence of intralocus gene conversion and recombination in a single-locus system[J]. Gene, 2012 , 497(1):52-57.
[14] Faulkes CG, Abbott DH, Mellor AL.Investigation of genetic diversity in wild colonies of naked mole-rats (Heterocephafus gfaber) by DNA fingerprinting[J]. J Zoology, 1990, 221(1): 87-97.
[15] Gladyshev VN, Zhang G, Wang J.The naked mole rat genome:Understanding aging through genome analysis[J]. Aging, 2011, 3(12):11-24.