Abstract: Objective To examine the vaginal cytology of estrous cycle in Guangxi Bama mini-pig. Methods Twelve mini-pig were selected and divided into different groups-diestrus group, proestrus group and estrus group. Vaginal smears of the pigs were obtained and stained with hematologic stain and Papanicolaou stain respectively .Epithelial cell morphology was observed and counted under the optical microscope. Results Leukocytes, para basal cell, small intermediate cell, large intermediate cell and superficial cell were found in vaginal smears. Leukocyte percentage was significantly higher during diestrus than that during estrus or proestrus (P<0.01). Para basal cell plus small intermediate cell counts were significantly higher during proestrus than that during estrus or diestrus (P<0.01). Superficial plus large intermediate cell counts were significantly higher during estrus than that during diestrus or proestrus (P<0.01). Conclusion Vaginal smears can be used to estimate different stage of estrous cycle in Guangxi Bama mini-pig.

Key words: Vaginal smear, Estrous cycle, Mini-pig