Application of PCR and Restiction Enzyme in Genotype Identification of uPA-SCID Mouse

doi:10.3969/j.issn.1674-5817.2015.03.008

Abstract

Abstract: Objective To set up a simple, fast and accurate method for the genotype identification of uPA-SCID mouse . Method The genotype of uPA-SCID mouse was detected by using PCR combined with restriction enzyme. Result The genotype identification results of uPA showed that the stripes of wild type in 153 bp, the stripes of homozygous in 337 bp, and the stripes of heterozygote in 337 bp and 153 bp. The genotype identification results of SCID showed that the mutant genes stripes in 38 bp and 26 bp, the heterozygote genes stripes in 64 bp, 38 bp and 26 bp, and the wild type stripes in 64 bp. Conclusion The method established is simple and accurate, it can identify the genotypes of uPA-SCID mouse, and screening the needful homozygous uPA-SCID mouse.

Key words: uPA-SCID mouse, Genotype identification, PCR, Restriction enzymes

CLC Number:

Q95-33

XU Chun-hua, CHEN Li-xiang, REN Xiao-nan, YANG Yu-qin, PENG Xiu-hua, CAI Jia-lin, ZHOU Xiao-hui, ZHOU Wen-jiang. Application of PCR and Restiction Enzyme in Genotype Identification of uPA-SCID Mouse[J]. Laboratory Animal and Comparative Medicine, 2015, 35(3): 218-221.

References

[1] 吴腾, 邢丽英, 勾红菊. ApoE-/-, SAP-Tg小鼠模型的构建[J].广东药学院学报, 2013, 29(05):539-542.
[2] 亓翠玲, 叶杰, 郭四美. Rip1-Tag2; PSGL-1-/-小鼠模型的构建[J]. 广东药学院学报, 2013, 29(1):76-78.
[3] Meuleman P, Libbrecht L, De Vos R, et a1. Morphological and biochemical characterization of a human liver in a uPA-SCID mouse chimera[J]. Hepatology, 2005, 41:847-856.
[4] 张海斌, 何志颖, 杨广顺. 人源化肝脏嵌合体小鼠的研究进展[J] . 中华肝脏病杂志, 2011, 19(5):398-400.
[5] Mercer DF, Schiller DE, Elliott JF, et al.Hepatitis C virus replication in mice with chimeric human livers[J], Nat Med, 2001, 7(8):927-933.
[6] 王廷华, 刘佳, 夏庆杰, 等. PCR理论和技术[M]. 北京: 科学出版社, 2013.
[7] 荣齐仙. 甘草药材中甘草酸含量变异的分子标记研究-rbcL、trnL内含子、IGS和matK[D]. 北京中医药大学,2007.
[8] 赵利伟, 胡朝晖, 陈文聪, 等. PCR-酶切技术快速鉴定军团菌[J]. 微生物学通报, 2013, 40(8):1514-1520.
[9] 曲良苗, 陈文炳, 缪婷玉, 等. 限制性内切酶酶切确证河豚鱼成分PCR检测结果[J]. 食品科学, 2014, 35(8):169-173.
[10] 王红, 金煜, 宋防. 限制性内切酶法在苯丙酮尿症突变基因诊断中的应用[J]. 中华医学检验杂志, 2008, 2, 31(2):186-187.
[11] 巫贵成, 周卫平. 乙型肝炎病毒感染的自然史研究进展[J]. 中华肝脏病杂志, 2001, 9(1):55-57.
[12] 刘石柱, 孙殿兴 . 乙型肝炎病毒PreSl的研究进展[J]. 国际病毒学杂志, 2012, 19(5):233-236.
[13] 刘义庆, 栾芳, 赵跃然, 等. 乙型肝炎病毒感染与肝外细胞及自身免疫病的研究进展[J] .中国医药, 2013, 8(10):1515-1517.
[14] 丁善龙, 王杰, 鲁凤民. 乙型肝炎研究及我国防治现状[J]. 传染病信息, 2013, 26(6):369-372.
[15] 徐培渝, Jerlin Walker.PCR 和限制性内切酶法快速检测C57BL/ 6J ob 小鼠基因型[J]. 癌变·畸变·突变, 2000,9(3):143-145.